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PE anti-mouse Galectin-9 Antibody
产品名称：
PE anti-mouse Galectin-9 Antibody
英文名称：
型号：
137903
产品库存：
1
产品价格：
电议

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Full length recombinant mouse Galectin-9 (M-type)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone 108A2 recognizes the linker peptide of mouse Galectin-9; it does not cross-react with stable Galectin-9 (which is biologically active, genetically engineered, linker-less Galectin-9)3. Additional reported applications (for the relevant formats) include: sandwich ELISA as capture antibody1 and immunohistochemical staining of paraffin2 embedded tissue sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Tsuboi Y, et al. 2007. Clin. Immunol. 124:221. (ELISA)
  2. Personal communication. (IHC-Paraffin)
  3. Oomizu S, et al. 2012. PLoS One. 7:e48574. PubMed
  4. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
Product Citations
  1. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
  2. Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed
  3. Knox T, et al. 2019. Sci Rep. 9:6136. PubMed
  4. Cao A, et al. 2018. Nat Commun. 9:3288. PubMed
  5. Smith LK, et al. 2021. Elife. 10:. PubMed
RRID
AB_10568785 (BioLegend Cat. No. 137903) AB_10567119 (BioLegend Cat. No. 137904)

Antigen Details

Structure
Homodimer, each chain is a 343 aa protein of 40 kD that has two conserved carbohydrate recognition domains (CRDs)
Distribution

Lymphocytes, dendritic cells, neutrophils, eosinophils, astrocytes, endothelial cells, fibroblasts, thymus stromal/epithelial cells

Function
Cell aggregation, cell adhesion, chemotaxis, apoptosis, suppression of Th1 and Th17 responses, induction of regulatory T cells
Ligand/Receptor
Tim-3
Cell Type
Astrocytes, Dendritic cells, Endothelial cells, Eosinophils, Epithelial cells, Fibroblasts, Lymphocytes, Neutrophils, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family
Adhesion Molecules, Immune Checkpoint Receptors
Antigen References

1. Klibi J, et al. 2009. Blood 113:1957.
2. Seki M, et al. 2008. Clin. Immunol. 127:78.
3. Tsuboi Y, et al. 2007. Clin. Immunol. 124:221.
4. Zhu C, et al. 2005. Nat. Immunol. 6:1245.
5. Dunphy JL, et al. 2002. J. Biol. Chem. 277:14916.

Gene ID
16859 View all products for this Gene ID
UniProt
View information about Galectin-9 on UniProt.org

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