Purified anti-human CD117 (c-kit) Antibody
- 产品名称：
- Purified anti-human CD117 (c-kit) Antibody
- 英文名称：
- 型号：
- 313201
- 产品库存：
- 1
- 产品价格：
- 电议
- Verified Reactivity
- Human
- Reported Reactivity
- Cynomolgus, Cow
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- MOLM-1 megakaryocytic cell line
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
CyTOF? - Verified
ICC, IP - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The 104D2 antibody does not block binding of c-Kit ligand. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy1, and spatial biology (IBEX)4,5.
- Application References
(PubMed link indicates BioLegend citation) -
- Broudy VC, et al. 1999. Blood 94:1979. (IF, IP)
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Nagano M, et al. 2007. Blood 110:151. (FC) PubMed
- Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
- Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
- Product Citations
-
- Lowinus T, et al. 2019. Cell Commun Signal. 17:05. PubMed
- Ortiz AM, et al. 2018. Nat Med. 24:1313. PubMed
- Vigneault P, et al. 2018. J Physiol. 596:2359. PubMed
- Vivanco Gonzalez N, et al. 2022. STAR Protoc. 3:101280. PubMed
- Ga?án-Gómez I, et al. 2022. Nat Med. . PubMed
- Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. PubMed
- Baskar R, et al. 2022. Cell Rep Methods. 2:. PubMed
- Nagano M, et al. 2007. Blood . 110:151. PubMed
- Guo A, et al. 2011. J Pharmacol Exp Ther. 338:421. PubMed
- Ardain A, et al. 2019. Nature. 570:528. PubMed
- Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
- Crawford LB, et al. 2021. J Virol. 95:. PubMed
- Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
- Cytlak U, et al. 2020. Immunity. 53(2):353-370. PubMed
- Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
- Jokela TA, et al. 2018. Front Cell Dev Biol. 6:41. PubMed
- RRID
- AB_314980 (BioLegend Cat. No. 313201) AB_314981 (BioLegend Cat. No. 313202)
- Structure
- Growth factor receptor with tyrosine kinase activity, subclass III, approximately 145 kD
- Distribution
-
Pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, acute myeloid leukemic cells (AML)
- Function
- Growth factor receptor for stem cell factor. Induces proliferation and survival of primitive hematopoietic progenitors. Potent inducer of proliferation in erythroid-committed progenitor cells. Defects in CD117 have been linked to severe anemia and a decreased number of hematopoietic progenitor cells.
- Ligand/Receptor
- c-Kit ligand
- Modification
- Multiple phosphorylation sites
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Giebel LB, et al. 1992. Oncogene 7:2207.
2. Furitsu T, et al. 1993. J. Clin. Invest. 92:1736.
- Gene ID
- 3815 View all products for this Gene ID
- UniProt
- View information about CD117 on UniProt.org
