PE/Cyanine7 anti-human CD117 (c-kit) Antibody
- 产品名称：
- PE/Cyanine7 anti-human CD117 (c-kit) Antibody
- 英文名称：
- 型号：
- 313211
- 产品库存：
- 1
- 产品价格：
- 电议
- Verified Reactivity
- Human
- Reported Reactivity
- Cynomolgus, Cow
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- MOLM-1 megakaryocytic cell line
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
The 104D2 antibody does not block binding of c-Kit ligand. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy1, and spatial biology (IBEX)4,5.
- Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Broudy VC, et al. 1999. Blood 94:1979. (IF, IP)
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Nagano M, et al. 2007. Blood 110:151. (FC) PubMed
- Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
- Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
- Product Citations
-
- Penkava F, et al. 2020. Nat Commun. 3.76875. PubMed
- Fang F, et al. 2020. Front Cell Dev Biol. 0.633333333. PubMed
- Thomas A, et al. 2021. Cancer Cell. 39(4):566-579.e7. PubMed
- Skovdahl HK, et al. 2021. Frontiers in Pharmacology. 12:679741. PubMed
- Grove K, et al. 2016. PLoS One. 11: 0145961. PubMed
- Liu S, et al. 2021. Mol Ther. 29:1918. PubMed
- Allan J, et al. 2017. Leuk Lymphoma. 10.1080/10428194.2017.1352089. PubMed
- Kerr BA, et al. 2021. Experimental Cell Research. 399(2):112456. PubMed
- Silver J, et al. 2020. Methods Mol Biol. 2121:115. PubMed
- Liao Y, et al. 2018. Stem Cells Transl Med. 0.659722222. PubMed
- Omer OS, et al. 2020. Methods Mol Biol. 2121:199. PubMed
- Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed
- Sicklinger F, et al. 2021. J Clin Invest. 131:. PubMed
- Kato M, et al. 2021. PLoS One. 16:e0252116. PubMed
- Gall M, et al. 2015. Mol Cancer Ther. 14: 2595 - 2605. PubMed
- Blomme EE, et al. 2021. Clin Transl Immunology. 10:e1287. PubMed
- Ogaki S, et al. 2015. Sci Rep. 5:17297. PubMed
- Serr I, et al. 2016. Nat Commun. 7:10991. PubMed
- RRID
- AB_893228 (BioLegend Cat. No. 313211) AB_893222 (BioLegend Cat. No. 313212)
- Structure
- Growth factor receptor with tyrosine kinase activity, subclass III, approximately 145 kD
- Distribution
-
Pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, acute myeloid leukemic cells (AML)
- Function
- Growth factor receptor for stem cell factor. Induces proliferation and survival of primitive hematopoietic progenitors. Potent inducer of proliferation in erythroid-committed progenitor cells. Defects in CD117 have been linked to severe anemia and a decreased number of hematopoietic progenitor cells.
- Ligand/Receptor
- c-Kit ligand
- Modification
- Multiple phosphorylation sites
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Giebel LB, et al. 1992. Oncogene 7:2207.
2. Furitsu T, et al. 1993. J. Clin. Invest. 92:1736.
- Gene ID
- 3815 View all products for this Gene ID
- UniProt
- View information about CD117 on UniProt.org
