APC anti-human CD117 (c-kit) Antibody
- 产品名称：
- APC anti-human CD117 (c-kit) Antibody
- 英文名称：
- 型号：
- 313205
- 产品库存：
- 1
- 产品价格：
- 电议
- Verified Reactivity
- Human
- Reported Reactivity
- Cynomolgus, Cow
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- MOLM-1 megakaryocytic cell line
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
The 104D2 antibody does not block binding of c-Kit ligand. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy1, and spatial biology (IBEX)4,5.
- Application References
(PubMed link indicates BioLegend citation) -
- Broudy VC, et al. 1999. Blood 94:1979. (IF, IP)
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Nagano M, et al. 2007. Blood 110:151. (FC) PubMed
- Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
- Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
- Product Citations
-
- Qiu J, et al. 2020. Cell Rep. 33:108465. PubMed
- Gordon E, et al. 2016. Proc Natl Acad Sci U S A. 113: 8765 - 8770. PubMed
- Zhan CS, et al. 2020. FASEB J. 14006:34. PubMed
- Rao S, et al. 2021. Cell Stem Cell. 28(5):833-845.e5. PubMed
- Lu B, et al. 2018. Cancer Cell. 34:970. PubMed
- Imai Y, et al. 2021. JID Innov. 1:100003. PubMed
- Frazier T, et al. 2018. Adipocyte. 0.41875. PubMed
- Leibel SL, et al. 2022. iScience. 25:103797. PubMed
- Mandl M, et al. 2014. PLoS One. 9:112140. PubMed
- Ruan S, et al. 2020. Front Pharmacol. 11:150. PubMed
- Sputova K, et al. 2013. Genome Integr. 29:4. PubMed
- Ran L, et al. 2018. Cancer Discov. 8:234. PubMed
- Luni C, et al. 2011. Biotechnol Prog. 27:1154. PubMed
- Shalabi SF, et al. 2021. Nat Aging. 1:838. PubMed
- Raman S, et al. 2021. Cell Reports Medicine. 2(7):100344. PubMed
- Zou T, et al. 2019. Nat Commun. 10:1205. PubMed
- He H, et al. 2019. J Cell Mol Med. 23:4139. PubMed
- Engeroff P, et al. 2020. J Allergy Clin Immunol. 145:301. PubMed
- Keck S, et al. 2021. Cellular and Molecular Gastroenterology and Hepatology. 12(2):507-545. PubMed
- RRID
- AB_314984 (BioLegend Cat. No. 313205) AB_314985 (BioLegend Cat. No. 313206)
- Structure
- Growth factor receptor with tyrosine kinase activity, subclass III, approximately 145 kD
- Distribution
-
Pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, acute myeloid leukemic cells (AML)
- Function
- Growth factor receptor for stem cell factor. Induces proliferation and survival of primitive hematopoietic progenitors. Potent inducer of proliferation in erythroid-committed progenitor cells. Defects in CD117 have been linked to severe anemia and a decreased number of hematopoietic progenitor cells.
- Ligand/Receptor
- c-Kit ligand
- Modification
- Multiple phosphorylation sites
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Giebel LB, et al. 1992. Oncogene 7:2207.
2. Furitsu T, et al. 1993. J. Clin. Invest. 92:1736.
- Gene ID
- 3815 View all products for this Gene ID
- UniProt
- View information about CD117 on UniProt.org
