Purified anti-human CD146 Antibody
- 产品名称：
- Purified anti-human CD146 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 361001
- 产品应用：
- 361001
- Verified Reactivity
- Human
- Reported Reactivity
- Mouse, Dog, Rabbit
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Cultured human umbilical cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F, WB - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats of this clone) include: Western blotting3 and IHC1,5.?
Clone P1H12 cross-reacts to Dog2, 5.
- Application References
(PubMed link indicates BioLegend citation) -
- Solovey A, et al. 1997. N. Engl. J. Med. 337:1584. (FC, IHC)
- Lamerato-Kozicki AR, et al. 2006. Exp. Hematol. 34:870. (FC)
- Balint K, et al. 2005. J. Clin. Invest. 115:3166. (WB)
- Neskey DM, et al. 2008. J. Exp. Clin. Cancer Res. 27:61. (ELISA)
- Kamstock D, et al. 2006. Cancer Gene Therap. 13:306. (IHC)
- Product Citations
-
- Pars S, et al. 2021. Cells. 10:. PubMed
- Stalin J, et al. 2016. Oncogene. 10.1038/onc.2016.83. PubMed
- RRID
- AB_2562882 (BioLegend Cat. No. 361001) AB_2562883 (BioLegend Cat. No. 361002)
- Structure
- 118 kD integral glycoprotein, Ig superfamily
- Distribution
-
Melanoma cells, epithelial cells, endothelial cells, activated T cells, multipotent mesenchymal stromal cells
- Function
- Adhesion, monocytes transendothelial migration
- Cell Type
- Endothelial cells, Epithelial cells, Mesenchymal cells, Mesenchymal Stem Cells, T cells
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Pickl WF, et al. 1997. J. Immunol. 158:2107.
2. Weninger W, et al. 2000. J. Invest. Dermatol. 115:219.
3. Sorrentino A, et al. 2008. Exp. Hematol. 36:1035.
4. Bardin N, et al. 2009. Arterioscler. Thromb. Vasc. Biol. 29:746.
- Gene ID
- 4162 View all products for this Gene ID
- UniProt
- View information about CD146 on UniProt.org
