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Purified anti-CD49e (Integrin α5) Antibody
产品名称:
Purified anti-CD49e (Integrin α5) Antibody
产品类别:
抗体
产品编号:
921704
产品应用:
921704
[价格]
规格 价格 库存
100µg ¥ 1488 1

产品详情

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
The P1D6 monoclonal antibody was generated against human HT1080 fibrosarcoma cells and reacts with the α5 integrin subunit.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 1.0 - 2.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats of this clone) include: blocking2. The LEAF? or Ultra-LEAF? purified antibody is recommended for blocking and functional assays (contact our custom solutions team).

This antibody binds to the cytoplasmic surface of native and denatured muscle AChR α1 subunits, within amino acids 371-386.


P1D6 interferes with binding of integrin-bearing cells to fibronectin but not collagen, laminin 1 or vitronectin coated surfaces.

This antibody neutralizes binding of α5β1 to the central cell adhesion domain of fibronectin, which is located within a 80 kD tryptic fragment.

Application References

(PubMed link indicates BioLegend citation)
  1. Wayner EA, et al. 1992. J. Cell. Biol. 116(2):489.
  2. Wayner EA, et al. 1987. J. Cell. Biol. 105:1873. (Block)
  3. Wayner EA, et al. 1989. J. Cell. Biol. 109(3):1321.
RRID
AB_2616796 (BioLegend Cat. No. 921704)

Antigen Details

Structure
Integrin α5 is a 115 kD protein.
Distribution
Plasma membrane.
Function
CD49e (Integrin α5) is a component of VLA-5 receptor. It is a cell surface adhesion receptor mediating cell-adhesion to extra cellular matrix or to other cells through heterodimerization and connecting to the cytoskeleton and various signaling molecules within the cells. Complexing with ITGB1 in fibronectin receptor.
Cell Type
Mesenchymal Stem Cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Gene ID
3678 View all products for this Gene ID
UniProt
View information about CD49e on UniProt.org

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