Alexa Fluor® 647 anti-SHIP-1 Antibody
- 产品名称：
- Alexa Fluor® 647 anti-SHIP-1 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 656607
- 产品应用：
- 656607
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human SHIP fusion protein in CFA/IFA
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Specific to human SHIP-1 and cross reactive with mouse SHIP-1.
- RRID
- AB_2563144 (BioLegend Cat. No. 656607) AB_2563145 (BioLegend Cat. No. 656608)
- Structure
- 1189 or 1188 amino acids, predicted molecular weight of 150 kD with the characteristic N-terminal SH2 domain, inositol phosphatase domain, and C-terminal protein interaction domains
- Distribution
- Cytoplasm and membrane; translocates to the plasma membrane when activated
- Function
- Negative regulator of hematopoietic cell proliferation and survival
- Interaction
- Phosphatidylinositol-3,4,5-triphosphate
- Ligand/Receptor
- SHC1, DOK1, DOK3, PTPN11/SHP-2, SLAMF1/CD150, PTPN11 FCGR2A FCGR2B, FCGR3, SRC and TEC et al.
- Cell Type
- B cells, Dendritic cells, Tregs
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Immunology, Innate Immunity, Signal Transduction
- Molecular Family
- Protein Kinases/Phosphatase
- Antigen References
-
1. Condé C, et al. 2011. Biochem. Pharmacol. 82:1320.
2. Parry RV, et al. 2010. Biochim Biophys Acta. 1804:592.
3. Ghansah T, et al. 2012. Oncoimmunology. 1:984.
4. Banh C, et al. 2012. Blood 120:4583.
5. Blunt MD, et al. 2012. Front. Immunol. 3:226.
- Gene ID
- 3635 View all products for this Gene ID
- UniProt
- View information about SHIP-1 on UniProt.org
