Purified anti-α-Synuclein Phospho (Ser129) A-上海雅吉生物科技有限公司

规格	价格	库存
25µL	￥ 1548	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

This monoclonal antibody was raised against a synthetic peptide corresponding to amino acids 124 - 134 of α-synuclein, phosphorylated at Serine 129, and conjugated to KLH via a C-terminal Cysteine.

Formulation

Phosphate-buffered solution with 0.1% azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

IHC-P?- Quality tested
ICC, IHC-F, WB -?Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1 - 2 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunohistochemistry (IHC-P). Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemisty on?frozen tissue sections (IHC-F),?and immunocytochemistry.

P-syn/81A is a mAb that is specific to alpha synuclein that has been phosphorylated on serine 129.

Application References (PubMed link indicates BioLegend citation)

Waxman EA, Giasson BI. 2008.?J. Neuropathol. Exp. Neurol.?67(5):402-16.?(IHC-P, WB)
Lim Y,?et al.?2011.?J. Neurosci.?31:10076.?(IHC-P)
Volpicelli-Daley LA,?et al.?2014.?Mol. Biol. Cell.?25:4010.?(ICC)?PubMed
Adamowicz DH,?et al. 2017.?J. Neurosci.?37(7):1675-1684.?(IHC-P, IHC-F)?PubMed

Product Citations

Yan M, et al. 2022. NPJ Parkinsons Dis. 8:1. PubMed
Dastidar SG, et al. 2020. J Neurosci. 8734:40. PubMed
Schaser AJ, et al. 2019. Sci Rep. 9:10919. PubMed
Creed RB, et al. 2020. Neuroscience. 437:64. PubMed
Doppler K, et al. 2016. Brain. 139: e5. PubMed
Volpicelli-Daley L, et al. 2014. Nat Protoc. 9:2135-2146. PubMed
Kim H, et al. 2022. Int J Mol Sci. 23:. PubMed
Kim S, et al. 2019. Neuron. 103(4):627-641.e7. PubMed
Adamowicz DH et al. 2017. The Journal of Neuroscience. 37(7):1675-1684 . PubMed
Zhang C, et al. 2021. Redox Biol. 47:102134. PubMed
Bottani E, et al. 2017. Mol Cell. 67:96. PubMed
Chung CC, et al. 2021. Biomolecules. 11: . PubMed
Lee J, et al. 2017. J Neuropathol Exp Neurol. 10.1093/jnen/nlw103. PubMed
Giannoccaro MP, et al. 2021. J Parkinsons Dis. 12:585. PubMed
Creed RB, et al. 2022. Acta Neuropathol Commun. 10:78. PubMed
Yoon YS, et al. 2022. Exp Mol Med. 54:115. PubMed
Park HE, et al. 2019. Adv Sci (Weinh). 6:1901673. PubMed
Bieri G, et al. 2019. Acta Neuropathol. 137:961. PubMed
Magalh?es P, et al. 2022. NPJ Parkinsons Dis. 8:93. PubMed
Ham S, et al. 2019. Int J Mol Sci. 4.639583333. PubMed
Breid S, et al. 2016. J Virol. 90: 9182 - 9193. PubMed
Zhu G, et al. 2020. Cell Rep. 108418:33. PubMed
Cai W et al. 2018. EBioMedicine. 29:13-22 . PubMed
Covell DJ, et al. 2017. Neuropathol Appl Neurobiol. 43:604. PubMed
Kim H, et al. 2020. Int J Mol Sci. 21:00. PubMed
Yuan X, et al. 2022. Front Neurosci. 15:807988. PubMed
Ryan T, et al. 2018. Nat Commun. 9:817. PubMed
Groveman BR et al. 2018. Acta neuropathologica communications. 6(1):7 . PubMed
Sampson TR et al. 2016. Cell. 167(6):1469-1480 . PubMed
Stoyka LE, et al. 2020. Neurobiol Dis. 134:104708. PubMed
Stykel MG, et al. 2021. Cell Reports. 35(6):109099. PubMed
Hu D, et al. 2019. Acta Neuropathol. 137:939. PubMed

RRID

AB_2734593 (BioLegend Cat. No. 825702) AB_2564891 (BioLegend Cat. No. 825701)

Antigen Details

Structure

α-synuclein's canonical isoform consists of 140 amino acids, which consist of four 11-residue repeats containing the consensus sequence KTKEGV. α-synuclein has an apparent molecular mass of 14 kD.

Distribution

Tissue distribution: primarily nervous system, but lower expression in other tissues such as skin.
Cellular distribution: cytoskeleton, cytosol, lysosome, mitochondria, nucleus, plasma membrane, and extracellular.

Function

The function of α-synuclein in the healthy brain is currently unknown.

Biology Area

Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation

Molecular Family

α-Synuclein, Phospho-Proteins

Antigen References