Alexa Fluor® 594 anti-α-Synuclein Phosph-上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1836	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

This monoclonal antibody was raised against a synthetic peptide corresponding to amino acids 124 - 134 of α-synuclein, phosphorylated at Serine 129, and conjugated to KLH via a C-terminal Cysteine.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 594 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Application Notes

This antibody is effective in immunohistochemistry (IHC-P). Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemisty on?frozen tissue sections (IHC-F),?and immunocytochemistry.

P-syn/81A is a mAb that is specific to alpha synuclein that has been phosphorylated on serine 129.

Application References (PubMed link indicates BioLegend citation)

Waxman EA, Giasson BI. 2008.?J. Neuropathol. Exp. Neurol.?67(5):402-16.?(IHC-P, WB)
Lim Y,?et al.?2011.?J. Neurosci.?31:10076.?(IHC-P)
Volpicelli-Daley LA,?et al.?2014.?Mol. Biol. Cell.?25:4010.?(ICC)?PubMed
Adamowicz DH,?et al. 2017.?J. Neurosci.?37(7):1675-1684.?(IHC-P, IHC-F)?PubMed

RRID

AB_2721293 (BioLegend Cat. No. 825707) AB_2721294 (BioLegend Cat. No. 825708)

Antigen Details

Structure

α-synuclein’s canonical isoform consists of 140 amino acids, which consist of four 11-residue repeats containing the consensus sequence KTKEGV. α-synuclein has an apparent molecular mass of 14 kD.

Distribution

Tissue distribution: primarily nervous system, but lower expression in other tissues such as skin.
Cellular distribution: cytoskeleton, cytosol, lysosome, mitochondria, nucleus, plasma membrane, and extracellular.

Function

The function of α-synuclein in the healthy brain is currently unknown.

Biology Area

Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation

Molecular Family

α-Synuclein, Phospho-Proteins

Antigen References