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PerCP anti-rat CD8a Antibody
产品名称:
PerCP anti-rat CD8a Antibody
产品类别:
抗体
产品编号:
201712
产品应用:
201712
[价格]
规格 价格 库存
100µg ¥ 3564 1

产品详情

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
High molecular weight glycoproteins from rat thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.

Application Notes

The OX-8 antibody has been reported to partially block T cell responses, including mixed lymphocyte reactions and cytotoxic T cell responses, and to induce macrophage activation. Additional reported applications (for the relevant formats) include: immunohistochemistry1,2 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections, immunoprecipitation3, in vivo and in vitro blocking of T cell responses3, 4, macrophage stimulation5, and Western blotting3,6.

Application References

(PubMed link indicates BioLegend citation)
  1. Barclay AN. 1981. Immunology 42:593. (IHC)
  2. Wallgren AC, et al. 1995. Transplantation 60:594. (IHC)
  3. Torres-Nagel N, et al. 1992. Eur. J. Immunol. 22:2841. (IP, WB)
  4. Mason DW, et al. 1983. Immunol. Rev. 74:57.
  5. Hirji N, et al. 1997. J. Immunol. 158:1833. (FA)
  6. Mitnacht R,, et al. 1998. J. Immunol. 160:700. (WB)
Product Citations
  1. Wang Y, et al. 2021. Front Cell Dev Biol. 9:702890. PubMed
  2. Chen C, et al. 2018. J Neuroinflammation. 15:167. PubMed
  3. Yanai H, et al. 2022. Elife. 11:. PubMed
  4. Li B, et al. 2020. Cell Mol Immunol. 1.386111111. PubMed
  5. Huang Z, et al. 2021. Food Chem Toxicol. 152:112239. PubMed
  6. Ruppert KA, et al. 2018. Sci Rep. 8:480. PubMed
RRID
AB_2075263 (BioLegend Cat. No. 201712)

Antigen Details

Structure
Ig superfamily, approximately 32 kD
Distribution

Thymocytes, NK cells, most CD8+ cells, intestinal intraepithelial lymphocytes, activated CD4+ cells, macrophages

Function
T cell activation, co-receptor for T cell activation
Ligand/Receptor
MHC class I molecule
Cell Type
Lymphocytes, Macrophages, NK cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Johnson P, et al. 1985. EMBO J. 4:2539.
2. Thomas ML, et al. 1983. Eur. J. Immunol. 13:855.

Gene ID
24930 View all products for this Gene ID
UniProt
View information about CD8a on UniProt.org

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