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Alexa Fluor® 647 anti-rat CD8a Antibody
产品名称:
Alexa Fluor® 647 anti-rat CD8a Antibody
产品类别:
抗体
产品编号:
201710
产品应用:
201710
[价格]
规格 价格 库存
100µg ¥ 3180 1

产品详情

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
High molecular weight glycoproteins from rat thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. For immunohistochemistry staining on frozen tissue sections, a concentration range of 2.5 - 10 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

The OX-8 antibody has been reported to partially block T cell responses, including mixed lymphocyte reactions and cytotoxic T cell responses, and to induce macrophage activation. Additional reported applications (for the relevant formats) include: immunohistochemistry1,2 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections, immunoprecipitation3, in vivo and in vitro blocking of T cell responses3, 4, macrophage stimulation5, and Western blotting3,6.

Application References

(PubMed link indicates BioLegend citation)
  1. Barclay AN. 1981. Immunology 42:593. (IHC)
  2. Wallgren AC, et al. 1995. Transplantation 60:594. (IHC)
  3. Torres-Nagel N, et al. 1992. Eur. J. Immunol. 22:2841. (IP, WB)
  4. Mason DW, et al. 1983. Immunol. Rev. 74:57.
  5. Hirji N, et al. 1997. J. Immunol. 158:1833. (FA)
  6. Mitnacht R,, et al. 1998. J. Immunol. 160:700. (WB)
RRID
AB_756150 (BioLegend Cat. No. 201710)

Antigen Details

Structure
Ig superfamily, approximately 32 kD
Distribution

Thymocytes, NK cells, most CD8+ cells, intestinal intraepithelial lymphocytes, activated CD4+ cells, macrophages

Function
T cell activation, co-receptor for T cell activation
Ligand/Receptor
MHC class I molecule
Cell Type
Lymphocytes, Macrophages, NK cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Johnson P, et al. 1985. EMBO J. 4:2539.
2. Thomas ML, et al. 1983. Eur. J. Immunol. 13:855.

Gene ID
24930 View all products for this Gene ID
UniProt
View information about CD8a on UniProt.org

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