Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 ?g per 10⁶ cells in 100 ?l. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

The OX-7 antibody reacts with rat CD90 and mouse CD90.1 (Thy-1.1) (which is expressed by mouse strains of AKR/J, PL, and FVB/N), but not mouse CD90.2.

Additional reported applications (for the relevant formats) include: immunohistochemical⁷ and immunofluorescent⁸ staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, immunoprecipitation¹, Western blotting¹, in vitro activation of leukocytes², induction of endothelial cell permeability³, induction of apoptosis in glomerular mesangial cells, and induction of glomerulonephritis in vivo⁴.

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

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8. Li B, et al. 2006. Kidney Int. 69:323. (ICC)
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10. Inagi R, et al. 2008. J Am Soc Nephrol. 19(5):915-22. (IHC-P)

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Hematopoietic stem cells, thymocytes, a subset of peripheral blood T cells, early myeloid and erythroid progenitors, immature B cells, neurons, activated endothelium, mast cells, dendritic cells, glomeruli

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2. Hosseinzadeh H, et al. 1993. J. Immunol. 150:1670.