Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. For immunohistochemistry on?frozen tissue sections, a concentration range of 5.0?- 10 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

The OX-7 antibody reacts with rat CD90 and mouse CD90.1 (Thy-1.1) (which is expressed by mouse strains of AKR/J, PL, and FVB/N), but not mouse CD90.2.

Additional reported applications (for the relevant formats) include: immunohistochemical⁷ and immunofluorescent⁸ staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, immunoprecipitation¹, Western blotting¹, in vitro activation of leukocytes², induction of endothelial cell permeability³, induction of apoptosis in glomerular mesangial cells, and induction of glomerulonephritis in vivo⁴.

1. Jeng CJ, et al. 1998. J. Cell Biol. 140:685. (IP, WB)
2. Nakashima I, et al. 1991. J. Immunol. 147:1153.
3. Ishizu A, et al. 1995. Int. Immunol. 7:1939.
4. Eitner F. 1997. Kidney. Int. 51:69.
5. Kawachi H, et al. 1992. Clin. Exp. Immunol. 88:399. (WB)
6. Dyer KD, et al. 2007. J. Immunol. 179:1693. (FC) PubMed
7. Daniel C, et al. 2012. Lab Invest. 92:812. (IHC-P)
8. Li B, et al. 2006. Kidney Int. 69:323. (ICC)
9. Uchimura H, et al. 2005. J Am Soc Nephrol. 16(4):997-1004. (IHC-F)
10. Inagi R, et al. 2008. J Am Soc Nephrol. 19(5):915-22. (IHC-P)

1. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
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4. Yamada T, et al. 2016. J Immunol. 197: 4079 - 4089. PubMed
5. Overacre-Delgoffe AE, et al. 2017. Cell. 169:1130. PubMed
6. Young R, et al. 2009. Blood. 114:5016. PubMed
7. Beura LK, et al. 2018. Immunity. 48:327. PubMed
8. Toshiro Hirai et al. 2019. Immunity. 50(5):1249-1261 . PubMed
9. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
10. Glodde N et al. 2017. Immunity. 47(4):789-802 . PubMed
11. Brede C, et al. 2012. J Clin Invest. 122:4439. PubMed

Hematopoietic stem cells, thymocytes, a subset of peripheral blood T cells, early myeloid and erythroid progenitors, immature B cells, neurons, activated endothelium, mast cells, dendritic cells, glomeruli

1. Campbell DG, et al. 1981. Biochem. J. 195:15.
2. Hosseinzadeh H, et al. 1993. J. Immunol. 150:1670.