Alexa Fluor® 594 anti-rat CD90/mouse CD90.1 (T-上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1488	1

Product Details

Verified Reactivity

Rat, Mouse

Reported Reactivity

Rabbit, Guinea Pig

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Rat thymocyte Thy-1 antigen

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 594 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

IHC-F - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 5.0 - 10 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Application Notes

The OX-7 antibody reacts with rat CD90 and mouse CD90.1 (Thy-1.1) (which is expressed by mouse strains of AKR/J, PL, and FVB/N), but not mouse CD90.2.

Additional reported applications (for the relevant formats) include: immunohistochemical⁷ and immunofluorescent⁸ staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, immunoprecipitation¹, Western blotting¹, in vitro activation of leukocytes², induction of endothelial cell permeability³, induction of apoptosis in glomerular mesangial cells, and induction of glomerulonephritis in vivo⁴.

Application References (PubMed link indicates BioLegend citation)

Jeng CJ, et al. 1998. J. Cell Biol. 140:685. (IP, WB)
Nakashima I, et al. 1991. J. Immunol. 147:1153.
Ishizu A, et al. 1995. Int. Immunol. 7:1939.
Eitner F. 1997. Kidney. Int. 51:69.
Kawachi H, et al. 1992. Clin. Exp. Immunol. 88:399. (WB)
Dyer KD, et al. 2007. J. Immunol. 179:1693. (FC) PubMed
Daniel C, et al. 2012. Lab Invest. 92:812. (IHC-P)
Li B, et al. 2006. Kidney Int. 69:323. (ICC)
Uchimura H, et al. 2005. J Am Soc Nephrol. 16(4):997-1004. (IHC-F)
Inagi R, et al. 2008. J Am Soc Nephrol. 19(5):915-22. (IHC-P)

Product Citations

Hakamivala A, et al. 2020. Bioact Mater. 5:142. PubMed

RRID

AB_2783139 (BioLegend Cat. No. 202545) AB_2783140 (BioLegend Cat. No. 202546)

Antigen Details

Structure: Ig superfamily, GPI-anchored membrane glycoprotein, 28-30 kD
Distribution: Hematopoietic stem cells, thymocytes, a subset of peripheral blood T cells, early myeloid and erythroid progenitors, immature B cells, neurons, activated endothelium, mast cells, dendritic cells, glomeruli
Function: Adhesion, signal transduction, lymphocyte co-stimulation, proliferation and differentiation of hematopoietic stem cells
Ligand/Receptor: Interacts with CD45
Cell Type: B cells, Dendritic cells, Endothelial cells, Hematopoietic stem and progenitors, Mast cells, Neurons, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Campbell DG, et al. 1981. Biochem. J. 195:15.
2. Hosseinzadeh H, et al. 1993. J. Immunol. 150:1670.
Gene ID: 21838 View all products for this Gene ID 24832 View all products for this Gene ID
UniProt: View information about CD90/CD90.1 on UniProt.org

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