FITC anti-rat CD103 (αE Integrin) Antibody
- 产品名称：
- FITC anti-rat CD103 (αE Integrin) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 205505
- 产品应用：
- 205505
- Verified Reactivity
- Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Density gradient enriched lymph veiled cells from PVG rats
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen skin and intestine epithelium1,4, immunocytochemical staining of cytospin preparations4, and Western blot1.
- Application References
(PubMed link indicates BioLegend citation) -
- Brenan M, et al. 1992. J. Exp. Med. 175:1457. (IHC, WB)
- Chen-Woan M, et al. 1995. J. Immunol. Methods 178:157.
- Chen-Woan M, et al. 1996. J. Leuk. Biol. 59:196.
- Brenan M and Rees DJ. 2000. Eur. J. Immunol. 30:1527. (IHC)
- Product Citations
-
- Li J, et al. 2022. Am J Transl Res. 14:5003. PubMed
- Li B, et al. 2019. Mol Med Rep. 19:5424. PubMed
- Morishita K, et al. 2015. Am J Physiol Gastrointest Liver Physiol. 309: G202-G208. PubMed
- RRID
- AB_10680460 (BioLegend Cat. No. 205505)
- Structure
- Single pass transmembrane protein, composed by two chains, heavy and light, linked by a disulfide bond. Associates with integrin β7 to form the αE/β7 complex.
- Distribution
-
Intraepithelial T lymphocytes, effector and memory Tregs, dendritic cells and their immediate precursors.
- Function
- Mediates adhesion of intraepithelial T cells (IEL) to epithelium.
- Ligand/Receptor
- E-cadherin
- Cell Type
- Dendritic cells, T cells, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Varas A, et al. 1998. Blood 92:93.
2. Brissette-Storkus CS, et al. 2002. J. Leukoc. Biol. 71:941.
3. Hubert FX, et al. 2004. J. Immunol. 172:7485.
4. Tsuchihashi S, et al. 2006. J. Immunol. 176:616.
5. Lührmann A, et al. 2007. Immunol. Lett. 108:167.
- Gene ID
- 83577 View all products for this Gene ID
- UniProt
- View information about CD103 on UniProt.org
