Biotin anti-rat CD103 (αE Integrin) Antibody-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1836	1

Product Details

Verified Reactivity

Rat

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Density gradient enriched lymph veiled cells from PVG rats

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen skin and intestine epithelium^1,4, immunocytochemical staining of cytospin preparations⁴, and Western blot¹.

Application References (PubMed link indicates BioLegend citation)

Brenan M, et al. 1992. J. Exp. Med. 175:1457. (IHC, WB)
Chen-Woan M, et al. 1995. J. Immunol. Methods 178:157.
Chen-Woan M, et al. 1996. J. Leuk. Biol. 59:196.
Brenan M and Rees DJ. 2000. Eur. J. Immunol. 30:1527. (IHC)

RRID

AB_10683166 (BioLegend Cat. No. 205503)

Antigen Details

Structure: Single pass transmembrane protein, composed by two chains, heavy and light, linked by a disulfide bond. Associates with integrin β7 to form the αE/β7 complex.
Distribution: Intraepithelial T lymphocytes, effector and memory Tregs, dendritic cells and their immediate precursors.
Function: Mediates adhesion of intraepithelial T cells (IEL) to epithelium.
Ligand/Receptor: E-cadherin
Cell Type: Dendritic cells, T cells, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Varas A, et al. 1998. Blood 92:93.
2. Brissette-Storkus CS, et al. 2002. J. Leukoc. Biol. 71:941.
3. Hubert FX, et al. 2004. J. Immunol. 172:7485.
4. Tsuchihashi S, et al. 2006. J. Immunol. 176:616.
5. Lührmann A, et al. 2007. Immunol. Lett. 108:167.
Gene ID: 83577 View all products for this Gene ID
UniProt: View information about CD103 on UniProt.org

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