Alexa Fluor® 647 anti-rat CD103 (αE Inte
- 产品名称：
- Alexa Fluor® 647 anti-rat CD103 (αE Inte
- 产品类别：
- 抗体
- 产品编号：
- 205509
- 产品应用：
- 205509
- Verified Reactivity
- Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Density gradient enriched lymph veiled cells from PVG rats
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen skin and intestine epithelium1,4, immunocytochemical staining of cytospin preparations4, and Western blot1.
- Application References
(PubMed link indicates BioLegend citation) -
- Brenan M, et al. 1992. J. Exp. Med. 175:1457. (IHC, WB)
- Chen-Woan M, et al. 1995. J. Immunol. Methods 178:157.
- Chen-Woan M, et al. 1996. J. Leuk. Biol. 59:196.
- Brenan M and Rees DJ. 2000. Eur. J. Immunol. 30:1527. (IHC)
- Product Citations
-
- Wu H, et al. 2016. Sci Rep. 6: 24548. PubMed
- RRID
- AB_10682895 (BioLegend Cat. No. 205509)
- Structure
- Single pass transmembrane protein, composed by two chains, heavy and light, linked by a disulfide bond. Associates with integrin β7 to form the αE/β7 complex.
- Distribution
-
Intraepithelial T lymphocytes, effector and memory Tregs, dendritic cells and their immediate precursors.
- Function
- Mediates adhesion of intraepithelial T cells (IEL) to epithelium.
- Ligand/Receptor
- E-cadherin
- Cell Type
- Dendritic cells, T cells, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Varas A, et al. 1998. Blood 92:93.
2. Brissette-Storkus CS, et al. 2002. J. Leukoc. Biol. 71:941.
3. Hubert FX, et al. 2004. J. Immunol. 172:7485.
4. Tsuchihashi S, et al. 2006. J. Immunol. 176:616.
5. Lührmann A, et al. 2007. Immunol. Lett. 108:167.
- Gene ID
- 83577 View all products for this Gene ID
- UniProt
- View information about CD103 on UniProt.org
