Alexa Fluor® 488 anti-rat CD25 Antibody
- 产品名称：
- Alexa Fluor® 488 anti-rat CD25 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 202108
- 产品应用：
- 202108
- Verified Reactivity
- Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Rat T cell blasts from mixed lymphocyte reactions
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemistryof acetone-fixed frozen sections1, immunoprecipitation1, weakly blocks IL-2 binding1, 2, 3, and blocks IL-2 induced epithelial cell migration3.
- Application References
(PubMed link indicates BioLegend citation) -
- Peterson DJ, et al. 1987. Mol. Immunol. 24:1281. (IHC, IP, Block)
- Tellides G, et al. 1987. Transplant Proc. 19:4231. (Block)
- Digness AU, et al. 1996. Exp.Cell Res. 225:422. (Block)
- Product Citations
-
- Chen KH, et al. 2019. J Neuropathol Exp Neurol. 78:65. PubMed
- RRID
- AB_2125458 (BioLegend Cat. No. 202108)
- Structure
- Type I membrane glycoprotein, 55 kD
- Distribution
-
Activated T cells and B cells, thymic and splenic dendritic cells, intestinal epithelial cells
- Function
- Low affinity receptor for IL-2; associates with IL-2 receptor β (CD122) and γ (CD132) chains to form high affinity receptor
- Ligand/Receptor
- IL-2
- Cell Type
- B cells, Dendritic cells, Epithelial cells, T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Digness AU, et al. 1996. Exp.Cell Res. 225:422.
2. Peterson DJ, et al. 1987. Mol. Immunol. 24:1281.
- Gene ID
- 16184 View all products for this Gene ID
- UniProt
- View information about CD25 on UniProt.org
