Alexa Fluor® 647 anti-rat CD4 (domain 2) Antib
- 产品名称：
- Alexa Fluor® 647 anti-rat CD4 (domain 2) Antib
- 产品类别：
- 抗体
- 产品编号：
- 203312
- 产品应用：
- 203312
- Verified Reactivity
- Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- MLR generated rat T helper lymphocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
- Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
The OX-35 antibody has been shown to bind a different CD4 epitope than the W3/25 and OX-38 antibodies. Additional reported applications (for the relevant formats) include: immunohistochemical staining2.
- Application References
(PubMed link indicates BioLegend citation) -
- May E, et al. 2003. J. Immunol. 170:1099.
- Hansson AS, et al. 1999. J. Clin. Invest. 104:589. (IHC)
- RRID
- AB_1501178 (BioLegend Cat. No. 203312)
- Structure
- Ig superfamily, 55 kD
- Distribution
- Majority of thymocytes, T cell subset
- Function
- TCR co-receptor, T cell activation
- Ligand/Receptor
- MHC class II molecule
- Cell Type
- Thymocytes, T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. May E, et al. 2003. J. Immunol. 170:1099.
2. Hansson AS, et al. 1999. J Clin Invest. 104:589
- Gene ID
- 12504 View all products for this Gene ID
- UniProt
- View information about CD4 on UniProt.org
