Alexa Fluor® 488 anti-mouse CD147 Antibody
- 产品名称：
- Alexa Fluor® 488 anti-mouse CD147 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 123717
- 产品应用：
- 123717
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
IHC-F -?Quality tested
3D IHC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.5 - 5.0 ?g/mL?is suggested.?For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Clone OX-114 has been shown to bind both two and three domain forms of mouse CD1471.
- Application References
(PubMed link indicates BioLegend citation) -
- Hanna SM,?et al.?2003.?BMC Biochemistry?4:17.
- Product Citations
-
- Okubo A, et al. 2021. Int J Mol Sci. 23:. PubMed
- RRID
- AB_2734189 (BioLegend Cat. No. 123717) AB_2734190 (BioLegend Cat. No. 123718)
- Structure
- Single chain type I transmembrane 58 kd heavily glycosylated protein. N terminal extracellular part consists of two C2 type Ig-like domains which are highly glycosylated. Recently reported: isoform with an additional membrane-distal Ig-like domain.
- Distribution
-
CD147 is expressed at varying levels in many cells types, including haematopoietic, epithelial, and endothelial cells. Expressed on immature thymocytes and at varying levels on spleen cells , bone marrow cells and macrophages.
- Function
- CD147 is implicated in multiple functions, such as matrix metalloproteinase induction, cell adhesion, retinal cell development, HIV attachment, embryonic development and T cell activation.
- Ligand/Receptor
- cyclophilin
- Cell Type
- Macrophages, Thymocytes
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Hanna SM, et al. 2003. BMC Biochemistry 4:17
2. Yurchenko V, et al. 2005. Immunology 117:301
3. Renno T, et al. 2002. J. Immunol. 168:4946
- Gene ID
- 12215 View all products for this Gene ID
- UniProt
- View information about CD147 on UniProt.org
