PE/Cyanine7 anti-rat CD45 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1764	1

Product Details

Verified Reactivity

Rat

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Enriched glycoprotein fraction from Wistar rat thymocytes

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation¹, immunofluorescence microscopy (acetone fixed, ammonium-thiocyanate separated epidermal sheets)², immunohistochemistry of acetone³-, isopentane-⁴, PLP⁵-, and n-hexane⁶ fixed frozen sections and paraffin-embedded sections^7,8, and partial inhibition of NK cell lysis of syngeneic tumor cell lines¹.

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References (PubMed link indicates BioLegend citation)

Giezeman-Smits KM, et al. 1999. J. Immunol. 163:71. (IP)
Elbe A, et al. 1994. J. Invest. Dermatol. 102:74. (IF)
Kouwenhoven E, et al. 2001. Kidney Int. 59:1142. (IHC)
Martin A, et al. 1995 Clin. Exp. Immunol. 22:283. (IHC)
Sayegh MH, et al. 1995 J. Exp. Med. 181:186. (IHC)
Morioka Y, et al. 2000. Kidney Int. 60:2192. (IHC)
Ng YY, et al. 2005. Kidney Int. 94:S83. (IHC-P)
Huang XR, et al. 2003. JASN. 14:1738. (IHC-P)

Product Citations

Xie L, et al. 2014. J Immunol. 192:6009. PubMed
Fehrenbach DJ, et al. 2020. Exp Physiol. 105:864. PubMed
Martín-Fernández B, et al. 2016. PLoS One. 11: 0145946. PubMed
Abais-Battad JM, et al. 2021. Acta Physiol (Oxf). 232:e13662. PubMed
Maxwell JW, et al. 2020. J Virol. 94:. PubMed
Lin ZH, et al. 2018. Front Cell Neurosci. 0.729861111. PubMed
Alsheikh AJ, et al. 2020. Am J Physiol Renal Physiol. F982:318. PubMed
Dasinger JH, et al. 2021. Pregnancy Hypertens. 24:126. PubMed
Li B, et al. 2020. Cell Mol Immunol. 1.386111111. PubMed
McCarthy C, et al. 2014. PLoS One. 9:95762. PubMed
Alsheikh AJ, et al. 2019. Am J Physiol Regul Integr Comp Physiol. 317:R182. PubMed
Bhushan S, et al. 2015. J Immunol. 194:5455. PubMed
Wei ZJ, et al. 2019. Neural Regen Res. 14:1462. PubMed
de Vries RJ, et al. 2020. Sci Rep. 1.181944444. PubMed

RRID

AB_2174546 (BioLegend Cat. No. 202213) AB_2253306 (BioLegend Cat. No. 202214)

Antigen Details

Structure: Protein tyrosine phosphatase, type I transmembrane protein, 180-220 kD (multiple isoforms and differential glycosylation)
Distribution: All hematopoietic cells except erythrocytes and platelets
Function: T cell receptor and B cell receptor signal transduction
Ligand/Receptor: Galectin-1, CD2, CD3, CD4
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Inhibitory Molecules
Molecular Family: CD Molecules
Antigen References: 1. Sunderland CA, et al. 1979. Eur. J. Immunol. 9:155.
2. Woolett GR, et al. 1985. Eur. J. Immunol.. 15:168.
Gene ID: 24699 View all products for this Gene ID
UniProt: View information about CD45 on UniProt.org

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