Pacific Blue™ anti-rat CD45 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1128	1

Product Details

Verified Reactivity

Rat

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Enriched glycoprotein fraction from Wistar rat thymocytes

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Pacific Blue? under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC -?Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Violet Laser (405 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation¹, immunofluorescence microscopy (acetone fixed, ammonium-thiocyanate separated epidermal sheets)², immunohistochemistry of acetone³-, isopentane-⁴, PLP⁵-, and n-hexane⁶ fixed frozen sections and paraffin-embedded sections^7,8, and partial inhibition of NK cell lysis of syngeneic tumor cell lines¹.

Application References (PubMed link indicates BioLegend citation)

Giezeman-Smits KM, et al. 1999. J. Immunol. 163:71. (IP)
Elbe A, et al. 1994. J. Invest. Dermatol. 102:74. (IF)
Kouwenhoven E, et al. 2001. Kidney Int. 59:1142. (IHC)
Martin A, et al. 1995 Clin. Exp. Immunol. 22:283. (IHC)
Sayegh MH, et al. 1995 J. Exp. Med. 181:186. (IHC)
Morioka Y, et al. 2000. Kidney Int. 60:2192. (IHC)
Ng YY, et al. 2005. Kidney Int. 94:S83. (IHC-P)
Huang XR, et al. 2003. JASN. 14:1738. (IHC-P)

Product Citations

Li J, et al. 2022. Am J Transl Res. 14:5003. PubMed
Areny-Balagueró A, et al. 2022. Pharmaceutics. 14:. PubMed
Shimamura Y, et al. 2022. Commun Biol. 5:753. PubMed

RRID

AB_2721618 (BioLegend Cat. No. 202225) AB_2721619 (BioLegend Cat. No. 202226)

Antigen Details

Structure: Protein tyrosine phosphatase, type I transmembrane protein, 180-220 kD (multiple isoforms and differential glycosylation)
Distribution: All hematopoietic cells except erythrocytes and platelets
Function: T cell receptor and B cell receptor signal transduction
Ligand/Receptor: Galectin-1, CD2, CD3, CD4
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Inhibitory Molecules
Molecular Family: CD Molecules
Antigen References: 1. Sunderland CA, et al. 1979. Eur. J. Immunol. 9:155.
2. Woolett GR, et al. 1985. Eur. J. Immunol.. 15:168.
Gene ID: 24699 View all products for this Gene ID
UniProt: View information about CD45 on UniProt.org

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