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Alexa Fluor® 647 anti-rat CD45 Antibody
产品名称:
Alexa Fluor® 647 anti-rat CD45 Antibody
产品类别:
抗体
产品编号:
202211
产品应用:
202211
[价格]
规格 价格 库存
25µg ¥ 1212 1

产品详情

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Enriched glycoprotein fraction from Wistar rat thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy (acetone fixed, ammonium-thiocyanate separated epidermal sheets)2, immunohistochemistry of acetone3-, isopentane-4, PLP5-, and n-hexane6 fixed frozen sections and paraffin-embedded sections7,8, and partial inhibition of NK cell lysis of syngeneic tumor cell lines1.

Application References

(PubMed link indicates BioLegend citation)
  1. Giezeman-Smits KM, et al. 1999. J. Immunol. 163:71. (IP)
  2. Elbe A, et al. 1994. J. Invest. Dermatol. 102:74. (IF)
  3. Kouwenhoven E, et al. 2001. Kidney Int. 59:1142. (IHC)
  4. Martin A, et al. 1995 Clin. Exp. Immunol. 22:283. (IHC)
  5. Sayegh MH, et al. 1995 J. Exp. Med. 181:186. (IHC)
  6. Morioka Y, et al. 2000. Kidney Int. 60:2192. (IHC)
  7. Ng YY, et al. 2005. Kidney Int. 94:S83. (IHC-P)
  8. Huang XR, et al. 2003. JASN. 14:1738. (IHC-P)
Product Citations
  1. Vallant N, et al. 2021. Int J Mol Sci. 22:00. PubMed
  2. Bhagwani AR, et al. 2020. Sci Rep. 1.205555556. PubMed
  3. Chang Y, et al. 2020. J Neuroinflammation. 0.860416667. PubMed
  4. Ohgomori T, et al. 2021. Eur J Neurosci. 54:4740. PubMed
  5. Subramaniyan B, et al. 2020. Photochem Photobiol. 668:96. PubMed
  6. Maldonado-Lasuncion I, et al. 2021. Int J Mol Sci. 22:. PubMed
  7. Titus DJ, et al. 2018. Neurobiol Learn Mem. 148:38. PubMed
  8. Lee SW, et al. 2019. J Neuroinflammation. 16:27. PubMed
RRID
AB_572066 (BioLegend Cat. No. 202211) AB_572067 (BioLegend Cat. No. 202212)

Antigen Details

Structure
Protein tyrosine phosphatase, type I transmembrane protein, 180-220 kD (multiple isoforms and differential glycosylation)
Distribution

All hematopoietic cells except erythrocytes and platelets

Function
T cell receptor and B cell receptor signal transduction
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Sunderland CA, et al. 1979. Eur. J. Immunol. 9:155.
2. Woolett GR, et al. 1985. Eur. J. Immunol.. 15:168.

Gene ID
24699 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

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