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Purified anti-β-Tubulin Antibody
产品名称:
Purified anti-β-Tubulin Antibody
产品类别:
抗体
产品编号:
605101
产品应用:
605101
[价格]
规格 价格 库存
25µg ¥ 1212 1

产品详情

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length protein expressed in HEK293T cells transfected with human TUBB4 expression vector.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 ?g per ml (1:500-1:5000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Because of complete sequence homology, this clone is also predicted to recognize TUBB4A in higher eukaryotes, including rat, monkey, pig, bovine, and canine. This clone was generated using full-length recombinant TUBB4A as an immunogen. However, due to high sequence homology, this clone may also recognize other members of the ?-Tubulin family, notably TUBB2A, TUBB3, TUBB4B, and TUBB8. Because these ?-Tubulins have a similar molecular weight as TUBB4A, we do not recommend using this antibody as a TUBB4A-specific antibody. This clone was generated using a more defined immunogen (recombinant TUBB4A) than our existing ?-Tubulin clone, TU27/Tubulin (Cat. No. 903401), which was generated against taxol-assembled microtubules (mixed tubulins).

Product Citations
  1. Ferreira D, et al. 2019. Sci Rep. 9:3958. PubMed
RRID
AB_2728462 (BioLegend Cat. No. 605101) AB_2728463 (BioLegend Cat. No. 605102)

Antigen Details

Structure
TUBB4A is a 444 amino acid protein with a predicted molecular weight of 49.58 kD.
Distribution

β-Tubulins are ubiquitously expressed, with TUBB4A showing highest expression levels in brain and moderate expression in testis. TUBB4A shows low expression in other tissues.

Function
Cell migration, cell trafficking, mitosis, cilia and flagella motor dynamics.
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Microtubules
Antigen References
  1. Arai-Ichinoi N, et al. 2016. Hum. Genet. 135: 89.
  2. Romaniello R, et al. 2015. Brain Dev. 37: 273.
  3. Shimojima K, et al. 2015. Brain Dev. 37: 281.
  4. Pizzino A, et al. 2014. Neurology. 83: 898.
  5. Tamura D, et al. 2013. Cancer Med. 2: 144.
  6. Hersheson J, et al. 2013. Ann. Neurol. 73: 546.
Gene ID
10382 View all products for this Gene ID
UniProt
View information about beta-Tubulin on UniProt.org

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