Alexa Fluor® 488 anti-Vimentin Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1632	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Full length human vimentin produced in E. coli.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICC?-?Quality tested
ICFC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, the suggested use of this reagent is?0.2 -?2 ?g per mL. For intracellular flow cytometry using our True-Phos? Perm Buffer, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?L volume.It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Blue Laser (488 nm)

Application Notes

While this clone recognizes mouse Vimentin, we do not recommend its usage for western blot due to poor affinity of the antibody for the protein.?Additional reported applications for the relevant formats include: spatial biology (IBEX)^1,2.

Application References (PubMed link indicates BioLegend citation)

Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed

Product Citations

Jarosch S, et al. 2022. STAR Protoc. 3:101374. PubMed
Buljan M, et al. 2020. Molecular Cell. 79(3):504-520. PubMed
Nethisinghe S, et al. 2021. Int J Mol Sci. 22:. PubMed
Rodríguez E, et al. 2022. Commun Biol. 5:41. PubMed
Zhou WJ, et al. 2022. Sci Adv. 8:eabj2488. PubMed

RRID

AB_2650955 (BioLegend Cat. No. 677809)

Antigen Details

Structure: 466 amino acids with a predicted molecular weight of approximately 54 kD.
Distribution: Cytoplasm.
Function: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Interaction: HCV core protein, LGSN, SYNM, PLEC, SLC6A4, STK33, LARP6, RAB8B, TOR1A, TOR1AIP1, and BCAS3.
Cell Type: B cells, Mesenchymal Stem Cells, Neural Stem Cells, Neutrophils
Biology Area: Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: Intermediate Filaments
Antigen References: 1. Kidd ME, et al. 2014. Am. J. Respir. Cell Mol. Biol. 50:1.
2. Fuchs E, et al. 1994. Annu. Rev. Biochem. 63:345.
3. Zeisberg M, et al. 2009. J. Clin. Invest. 119:1429.
4. Scanlon CS, et al. 2013. J. Dent. Res. 92:114.
5. Thebault S, et al. 2002. J. Immunol. 169:4046.
6. Vossenaar ER, et al. 2004. Arthritis Res. Ther. 6:R142.
7. Rose ML. 2013. Hum. Immunol. 74:1459.
Gene ID: 7431 View all products for this Gene ID
UniProt: View information about Vimentin on UniProt.org

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