PE anti-human CD27 Antibody
- 产品名称：
- PE anti-human CD27 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 302807
- 产品应用：
- 302807
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Baboon, Squirrel Monkey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- ?g size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA). - Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- ?g sizes: 0.2 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the ?g size, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
- Product Citations
-
- Karlsson H, et al. 2015. PLoS One. 10: 0144787. PubMed
- Chen YQ et al. 2018. Cell. 173(2):417-429 . PubMed
- Chen F, et al. 2021. Immunity. 54(4):781-796.e4. PubMed
- Swanson E, et al. 2021. eLife. 10:00. PubMed
- Soriano-Sarabia N, et al. 2014. J Virol. 88:14070. PubMed
- Fu Y, et al. 2021. Cell Reports. 36(2):109344. PubMed
- Savage AK, et al. 2021. iScience. 24(5):102404. PubMed
- Wong GK, et al. 2019. JCI Insight. 4. PubMed
- Genge PC, et al. 2021. STAR Protoc. 2:100900. PubMed
- Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed
- Li X, et al. 2021. Front Immunol. 12:779560. PubMed
- RRID
- AB_314299 (BioLegend Cat. No. 302807) AB_314300 (BioLegend Cat. No. 302808) AB_2564146 (BioLegend Cat. No. 302842)
- Structure
- TNF-R superfamily, type I transmembrane glycoprotein, 50-55 kD
- Distribution
-
Medullary thymocytes, T and B cell subsets, NK cells
- Function
- T cell costimulation
- Ligand/Receptor
- CD70
- Cell Type
- B cells, NK cells, T cells, Thymocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Hintzen R, et al. 1994. Immunol. Today 15:307.
2. Agematsu K, et al. 1995. J. Immunol. 154:3627.
- Gene ID
- 939 View all products for this Gene ID
- UniProt
- View information about CD27 on UniProt.org
