Alexa Fluor® 700 anti-human CD27 Antibody
- 产品名称：
- Alexa Fluor® 700 anti-human CD27 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 302813
- 产品应用：
- 302813
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Baboon, Squirrel Monkey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
- Product Citations
-
- Ravens S, et al. 2020. Proc Natl Acad Sci U S A. 117:18649. PubMed
- Sauce D, et al. 2012. J Immunol. 189:5541. PubMed
- Esaulova E, et al. 2020. Cell Host Microbe. 29(2):165-178.e8. PubMed
- Eller MA, et al. 2016. J Virol. 90: 4005-4016. PubMed
- Fali T, et al. 2019. J Immunol. 202:1962. PubMed
- Onodera T, et al. 2019. J Immunol. 203:3282. PubMed
- Sayin I, et al. 2018. J Exp Med. 7:40286. PubMed
- Buggert M, et al. 2020. Cell. 183(7):1946-1961.e15. PubMed
- Wang X, et al. 2019. J Immunol. 203:2171. PubMed
- Rouers A, et al. 2021. iScience. 24:102482. PubMed
- Cherukuri A, et al. 2014. J Am Soc Nephrol. 7:1575. PubMed
- NULL, et al. 2022. Cell. 185:916. PubMed
- Adachi Y, et al. 2019. Nat Commun. 10:3883. PubMed
- Almeida J, et al. 2007. J Exp Med. 204:2473. PubMed
- Papagno L, et al. 2020. Sci Rep. 10:11620. PubMed
- Sauce D, et al. 2011. Blood. 117:5142. PubMed
- RRID
- AB_493756 (BioLegend Cat. No. 302813) AB_493757 (BioLegend Cat. No. 302814)
- Structure
- TNF-R superfamily, type I transmembrane glycoprotein, 50-55 kD
- Distribution
-
Medullary thymocytes, T and B cell subsets, NK cells
- Function
- T cell costimulation
- Ligand/Receptor
- CD70
- Cell Type
- B cells, NK cells, T cells, Thymocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Hintzen R, et al. 1994. Immunol. Today 15:307.
2. Agematsu K, et al. 1995. J. Immunol. 154:3627.
- Gene ID
- 939 View all products for this Gene ID
- UniProt
- View information about CD27 on UniProt.org
