PerCP/Cyanine5.5 anti-mouse CD63 Antibody
- 产品名称:
- PerCP/Cyanine5.5 anti-mouse CD63 Antibody
- 产品类别:
- 抗体
- 产品编号:
- 143911
- 产品应用:
- 143911
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Intestinal lamina propria light-density cells (enriched with eosinophils)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
FC -?Verified
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.5??g per million cells in 100 ?L volume. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?L?volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: Western blotting1 and immunofluorescence1.
- Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Verjan Garcia N, et al. 2011. J. Immunol. 187:2268. (WB, IF)
- RRID
- AB_2565501 (BioLegend Cat. No. 143911) AB_2565502 (BioLegend Cat. No. 143912)
- Structure
- Tetraspan transmembrane superfamily (TM4SF), type III lysosomal glycoprotein, 53 kD
- Distribution
-
Activated platelets, monocytes, macrophages, endothelium, fibroblasts, osteoclasts, and smooth muscle cells
- Function
- Platelet activation
- Interaction
- CD9, CD81, VLA-3, and VLA-6
- Cell Type
- Endothelial cells, Fibroblasts, Macrophages, Monocytes, Osteoclasts, Platelets
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
-
1. Azorsa DO, et al. 1991. Blood 78:280.
2. Kishimoto T, et al.?1997. Leukocyte Typing V1. Oxford University Press New York.
3. Hildreth JE, et al. 1991. Blood 77:121.
- Gene ID
- 12512 View all products for this Gene ID
- UniProt
- View information about CD63 on UniProt.org