PE/Dazzle™ 594 anti-mouse CD63 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1908	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Intestinal lamina propria light-density cells (enriched with eosinophils)

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with PE/Dazzle? 594 under optimal conditions.

Concentration

0.2 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested
FC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For?intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.5??g per million cells in 100 ?L volume. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?L?volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Dazzle? 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: Western blotting¹ and immunofluorescence¹.

Application References (PubMed link indicates BioLegend citation)

Verjan Garcia N, et al. 2011. J. Immunol. 187:2268. (WB, IF)

Product Citations

Milich LM, et al. 2021. J Exp Med. 218:. PubMed
Scheyltjens I, et al. 2022. Nat Protoc. 17:2354. PubMed

RRID

AB_2565503 (BioLegend Cat. No. 143913) AB_2565504 (BioLegend Cat. No. 143914)

Antigen Details

Structure: Tetraspan transmembrane superfamily (TM4SF), type III lysosomal glycoprotein, 53 kD
Distribution: Activated platelets, monocytes, macrophages, endothelium, fibroblasts, osteoclasts, and smooth muscle cells
Function: Platelet activation
Interaction: CD9, CD81, VLA-3, and VLA-6
Cell Type: Endothelial cells, Fibroblasts, Macrophages, Monocytes, Osteoclasts, Platelets
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Azorsa DO, et al. 1991. Blood 78:280.
2. Kishimoto T, et al.?1997. Leukocyte Typing V1. Oxford University Press New York.
3. Hildreth JE, et al. 1991. Blood 77:121.
Gene ID: 12512 View all products for this Gene ID
UniProt: View information about CD63 on UniProt.org

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