APC anti-mouse CD63 Antibody
- 产品名称：
- APC anti-mouse CD63 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 143905
- 产品应用：
- 143905
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Intestinal lamina propria light-density cells (enriched with eosinophils)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
FC -?Verified
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For?intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.5??g per million cells in 100 ?L volume. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?L?volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: Western blotting1 and immunofluorescence1.
- Application References
(PubMed link indicates BioLegend citation) -
- Verjan Garcia N, et al. 2011. J. Immunol. 187:2268. (WB, IF)
- Product Citations
-
- McPherson HR, et al. 2021. Elife. 10:. PubMed
- Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
- Ratitong B, et al. 2021. Cell Reports. 35(7):109139. PubMed
- Jiao Q, et al. 2020. Front Immunol. 11:469. PubMed
- López-Sanz C, et al. 2022. STAR Protoc. 3:101755. PubMed
- Sanz-Ros J, et al. 2022. Sci Adv. 8:eabq2226. PubMed
- Lv L, et al. 2021. Front Immunol. 12:675534. PubMed
- Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
- RRID
- AB_2565495 (BioLegend Cat. No. 143905) AB_2565496 (BioLegend Cat. No. 143906)
- Structure
- Tetraspan transmembrane superfamily (TM4SF), type III lysosomal glycoprotein, 53 kD
- Distribution
-
Activated platelets, monocytes, macrophages, endothelium, fibroblasts, osteoclasts, and smooth muscle cells
- Function
- Platelet activation
- Interaction
- CD9, CD81, VLA-3, and VLA-6
- Cell Type
- Endothelial cells, Fibroblasts, Macrophages, Monocytes, Osteoclasts, Platelets
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
-
1. Azorsa DO, et al. 1991. Blood 78:280.
2. Kishimoto T, et al.?1997. Leukocyte Typing V1. Oxford University Press New York.
3. Hildreth JE, et al. 1991. Blood 77:121.
- Gene ID
- 12512 View all products for this Gene ID
- UniProt
- View information about CD63 on UniProt.org
