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Purified anti-
产品名称:
Purified anti-
产品类别:
抗体
产品编号:
662904
产品应用:
662904
[价格]
规格 价格 库存
100µg ¥ 3876 1

产品详情

Product Details

Verified Reactivity
E. coli, Enterics, Borellia burgdorferi, Salmonella
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
1295-1417 (SLAELLNAGLGGS) of the E. coli RNA polymerase β subunit.
Formulation
Phosphate-buffered solution, pH 7.2.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ELISA, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone NT73 is extremely useful for purifying holoenzymes from enterics. It does not cross-react with Mycobacterial smegmatis RNAP. This antibody is used for purifying Softag 1 epitope tagged protein expressed in eukaryotic systems. Clone NT73 recognizes epitope?1295-1417 (SLAELLNAGLGGS).

Additional reported applications (for the relevant formats) include: immunoprecipitation.

Application References

(PubMed link indicates BioLegend citation)
  1. Thompson NE, et al. 1992. Biochemistry 31:7003.
  2. Thompson NE, et al. 2003. Anal. Biochem. 323:171.
RRID
AB_2566242 (BioLegend Cat. No. 662904)

Antigen Details

Structure
1252 amino acids with a predicted molecular weight of approximately 150 kD.
Distribution

Bacteria

Function
Transcriptional regulator in bacteria.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Thompson NE, et al. 1992. Biochemistry 31:7003.
2. Hurwitz J, et al. 2005. J. Biol. Chem. 280:42477.
3. Hirata A, et al. 2008. Nature 451:851.

Gene ID
948488 View all products for this Gene ID
UniProt
View information about RNA Polymerase beta Prime on UniProt.org

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