WB - Quality tested
ELISA, IP - Reported in the literature, not verified in house
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Clone NT73 is extremely useful for purifying holoenzymes from enterics. It does not cross-react with Mycobacterial smegmatis RNAP. This antibody is used for purifying Softag 1 epitope tagged protein expressed in eukaryotic systems. Clone NT73 recognizes epitope?1295-1417 (SLAELLNAGLGGS).
Additional reported applications (for the relevant formats) include: immunoprecipitation.
Bacteria
1. Thompson NE, et al. 1992. Biochemistry 31:7003.
2. Hurwitz J, et al. 2005. J. Biol. Chem. 280:42477.
3. Hirata A, et al. 2008. Nature 451:851.