Ultra-LEAF™ Purified anti-human CD352 (NTB-A
- 产品名称：
- Ultra-LEAF™ Purified anti-human CD352 (NTB-A
- 产品类别：
- 抗体
- 产品编号：
- 317211
- 产品应用：
- 317211
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant extracellular domain of NTB-A
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Block, Stim - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5??g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: stimulation of NTB-A in a redirected lysis assay and blocking of NTB-A function3. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 317211 & 317212). Co-stimulation of cells with CD3 antibodies and clone NT-7 has been shown to induce activation rather than blocking function of these cells3.
- Application References
(PubMed link indicates BioLegend citation) -
- Messmer B, et al. 2006. J. Immunol. 176:4646. (FC)
- Ward J, et al. 2007. Blood 110:1207. (FC) PubMed
- Uzana R, et al. 2012. J. Immunol. 188:632. (Block) PubMed
- Baglaenko Y, et al. 2017. J. Immunol. 198: 3949. (Stim)
- RRID
- AB_2860836 (BioLegend Cat. No. 317211) AB_2860837 (BioLegend Cat. No. 317212)
- Structure
- Type I transmembrane tyrosine-phosphorylated glycoprotein, CD2/CD150 subfamily, 60 kD
- Distribution
-
NK cells, T cells, B cells
- Function
- CD28 independent T cell costimulation, induction of NK cell cytotoxicity, regulation of B cell tolerance
- Ligand/Receptor
- NTBA
- Cell Type
- B cells, NK cells, T cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Bottino C, et al. 2001. J. Exp. Med. 194:235.
2. Valdez PA, et al. 2004. J. Bio. Chem. 279:18662.
3. Flaig RM, et al. 2004. J. Immunol. 172:6524.
4. Falco M, et al. 2004. Eur. J. Immunol. 34:1663.
5. Kumar KR, et al. 2006. Science 312:1665.
- Gene ID
- 114836 View all products for this Gene ID
- UniProt
- View information about CD352 on UniProt.org
