Alexa Fluor® 594 anti-NSE Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1488	1

Product Details

Verified Reactivity

Human, Mouse, Rat

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

This antibody was raised against a synthetic peptide corresponding to amino acids 416-433. It recognizes the sequence LGDEARFAGHNFRNPSVL.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 594 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

IHC-P - Quality tested
ICC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 ?g/ml is suggested.?For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Application Notes

The reactivity of Alexa Fluor? 647 and Alexa Fluor? 488 anti-NSE antibody was verified in FFPE mouse and rat tissue.

Additional reported applications (for the relevant formats) include: ELISA² and immunohistochemistry^1,3,4.

Application References (PubMed link indicates BioLegend citation)

Yoo J, et al. 2002. Arch. Pathol. Lab Med. 126(8):979. (IHC-P)
Duncan ME, et al. 1992. J. Immuno. Methods. 151:227 (ELISA)
Chetty R. 2000. Pathology. 32(3):209. (IHC-P)
Murray GI, et al. 1993. J. Clin. Pathol. 46:993. (IHC-P)

RRID

AB_2721395 (BioLegend Cat. No. 804902) AB_2721396 (BioLegend Cat. No. 804903)

Antigen Details

Structure

Gamma enolase is a 434 amino acid protein with a molecular mass of 47 kD.

Distribution

Tissue distribution: Nervous and musculoskeletal systems.
Cellular distribution: Secreted, cytosol, plasma membrane, cytoskeleton, and nucleus.

Function

Gamma enolase has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. It binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.

Cell Type

Mature Neurons, Neural Stem Cells, Neurons

Biology Area

Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells

Molecular Family

Enzymes and Regulators, Phospho-Proteins

Antigen References