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PE anti-human CD178 (Fas-L) Antibody
产品名称：
PE anti-human CD178 (Fas-L) Antibody
产品类别：
抗体
产品编号：
306406
产品应用：
306406
[价格]
规格 价格 库存
25tests ￥ 1750 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
L5178Y mouse T lymphoma cells expressing recombinant human FasL
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, immunofluorescence microscopy3, immunocytochemistry2, blocking of Fas induced apoptosis1, and Western blotting11. Fas Ligand is expressed at low density on activated cells. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 306407) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 306404) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)). In addition, applying matrix metalloproteinases (MMPS) inhibitor in the cell culture system will increase the FasL staining intensity. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 306415 and 306416).

Application References

(PubMed link indicates BioLegend citation)
  1. Kayagaki N, et al. 1995. J. Exp. Med. 182:1777.
  2. Herr I, et al. 2000. Cell Death Differ. 7:129. (WB)
  3. Bossi G, et al. 1999. Nature Medicine 5:90.
  4. Andreola G, et al. 2002. J. Exp. Med. 195:1303.
  5. Strauss L, et al. 2009. J. Immunol. 182:1469. PubMed
  6. Li JH, et al. 2009. Am J. Pathol. 175:1124. PubMed
  7. Zhao Q, et al. 2011. Fitoterapia. 82:735. PubMed
  8. Kruger K, et al. 2011. J. Appl. Physiol. 110:1226. PubMed
  9. Qin G, et al. 2012. J. Infect. Dis. PubMed
  10. Khalid M, et al. 2012. J. Virol. 86:4906. PubMed
  11. Qin G, et al. 2012. J. Infect. Dis. 205:1646. PubMed
  12. Shrestha B, et al. 2012. J. Virol. 86:8937. PubMed
  13. Mooren FC, et al. 2012. J. Appl. Physiol.113:1082. PubMed
  14. Robinet P, et al. 2014. J Immunol. 192:5332. PubMed
  15. Wang Y, et al. 2014. J Endocrinol. 222:151. PubMed
Product Citations
  1. Sulejmani O, et al. 2021. Cancers (Basel). 13:. PubMed
  2. Wang Y, et al. 2014. J Endocrinol. 222:151. PubMed
  3. Afolabi LO, et al. 2021. Front Immunol. 12:701671. PubMed
  4. Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed
  5. Fujii C, et al. 2016. Sci Rep. 6:35314. PubMed
  6. Contreras-Ochoa CO, et al. 2022. Iran J Basic Med Sci. 25:383. PubMed
  7. Hofmann L, et al. 2022. Front Med (Lausanne). 9:904295. PubMed
  8. Medina M, et al. 2012. J Leukoc Biol. 91:759. PubMed
  9. Felices M, et al. 2018. JCI Insight. 3. PubMed
  10. Wu J, et al. 2021. Adv Sci (Weinh). 8:e2101029. PubMed
  11. Van der Meer JMR, et al. 2021. Oncoimmunology. 10:1981049. PubMed
  12. Zhao Q, et al. 2011. Fitoterapia. 82:735. PubMed
  13. Meng Q, et al. 2021. J Immunother Cancer. 9:. PubMed
  14. Meggyes M, et al. 2021. Biomolecules. 11:. PubMed
  15. Sch?nberger S, et al. 2020. Cancers (Basel). 12:. PubMed
  16. Carrion B, et al. 2021. Neurol Neuroimmunol Neuroinflamm. 8:. PubMed
  17. Lake CM, et al. 2021. Cell Death Dis. 12:400. PubMed
  18. Robinet P, et al. 2014. J Immunol. 192:5332. PubMed
  19. Lamichhane R et al. 2019. Cell Rep. 28(12):3061-3076 . PubMed
RRID
AB_314603 (BioLegend Cat. No. 306406) AB_2100664 (BioLegend Cat. No. 306407)

Antigen Details

Structure
TNF superfamily, type II glycoprotein, 38-42 kD, 26 kD soluble form
Distribution

Activated T cells, NK cells, testis, eye, neutrophils, Clara type II cells

Function
Apoptosis, immune privilege
Ligand/Receptor
CD95
Cell Type
Neutrophils, NK cells, T cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Suda T, et al. 1997. J. Exp. Med. 12:204.
2. Kayagaki N, et al. 1995. J. Exp. Med. 182:1777.
3. Tanaka M, et al. 1995. EMBO J. 14:1129.

Gene ID
356 View all products for this Gene ID
UniProt
View information about CD178 on UniProt.org

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