APC/Fire™ 750 anti-mouse CD205 (DEC-205) Ant
- 产品名称：
- APC/Fire™ 750 anti-mouse CD205 (DEC-205) Ant
- 产品类别：
- 抗体
- 产品编号：
- 138219
- 产品应用：
- 138219
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse lymph node tissue
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC/Fire? 750 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* APC/Fire? 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1, Western Blot1-3, immunoprecipitation of bone marrow dendritic cell extracts2, and spatial biology (IBEX)6,7.
- Application References
(PubMed link indicates BioLegend citation) -
- Witmer-Pack MD, et al. 1995. Cell. Immun. 163:157. (IHC, WB)
- Swiggard WJ, et al. 1995. Cell. Immun. 165:302. (WB, IP)
- Bonifaz LC, et al. 2004. J. Exp. Med. 199:815. (WB)
- Yamazaki S, et al. 2002. J. Immunol. 181:6923. (FC)
- Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC)
- Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
- Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
- RRID
- AB_2800645 (BioLegend Cat. No. 138219) AB_2800646 (BioLegend Cat. No. 138220)
- Structure
- A 205 kD type I tramsmembrane protein belonging to the C-type lectin family and homologous to the macrophage mannose receptor.
- Distribution
-
Expressed at high levels by dendritic cells and thymic epithelial cells. Also expressed by B lymphocytes, macrophages, Langerhans cells, bone marrow cells and epithelial cells.
- Function
- Mediates endocytosis, antigen uptake, presentation, and cross-presentation to T cells.
- Cell Type
- B cells, Dendritic cells, Epithelial cells, Langerhans cells, Macrophages
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
-
1. Jiang WP, et al. 1995. Nature 375:151.
2. Small M and Kraal G. 2003. Int. Immunol. 15:197.
3. Shrimpton RE, et al. 2009. Mol. Immunol. 46:1229.
- Gene ID
- 17076 View all products for this Gene ID
- UniProt
- View information about CD205 on UniProt.org
