Alexa Fluor® 647 anti-mouse CD205 (DEC-205) An-上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1764	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse lymph node tissue

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

SB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections¹, Western Blot^1-3, immunoprecipitation of bone marrow dendritic cell extracts², and spatial biology (IBEX)^6,7.

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References (PubMed link indicates BioLegend citation)

Witmer-Pack MD, et al. 1995. Cell. Immun. 163:157. (IHC, WB)
Swiggard WJ, et al. 1995. Cell. Immun. 165:302. (WB, IP)
Bonifaz LC, et al. 2004. J. Exp. Med. 199:815. (WB)
Yamazaki S, et al. 2002. J. Immunol. 181:6923. (FC)
Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC)
Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

Product Citations

Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
Mambres D, et al. 2015. PLoS One. 10: 0137835. PubMed
Chmielewski M and Abken H 2017. Cell Rep.. 10.1016/j.celrep.2017.11.063. PubMed

RRID

AB_2297115 (BioLegend Cat. No. 138203) AB_2137655 (BioLegend Cat. No. 138204)

Antigen Details

Structure: A 205 kD type I tramsmembrane protein belonging to the C-type lectin family and homologous to the macrophage mannose receptor.
Distribution: Expressed at high levels by dendritic cells and thymic epithelial cells. Also expressed by B lymphocytes, macrophages, Langerhans cells, bone marrow cells and epithelial cells.
Function: Mediates endocytosis, antigen uptake, presentation, and cross-presentation to T cells.
Cell Type: B cells, Dendritic cells, Epithelial cells, Langerhans cells, Macrophages
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Jiang WP, et al. 1995. Nature 375:151.
2. Small M and Kraal G. 2003. Int. Immunol. 15:197.
3. Shrimpton RE, et al. 2009. Mol. Immunol. 46:1229.
Gene ID: 17076 View all products for this Gene ID
UniProt: View information about CD205 on UniProt.org

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