Ultra-LEAF™ Purified anti-human CD49e Antibo
- 产品名称：
- Ultra-LEAF™ Purified anti-human CD49e Antibo
- 产品类别：
- 抗体
- 产品编号：
- 328013
- 产品应用：
- 328013
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Baboon
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- U937 cells
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
IP, ELISA, Block - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0??g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and inhibition of a5 integrin mediated cell adhesion2. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 328004).
- Application References
(PubMed link indicates BioLegend citation) -
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Orecchia A, et al. 2003. J. Cell Sci. 116:3479. (Block)
- RRID
- AB_2860857 (BioLegend Cat. No. 328013) AB_2860858 (BioLegend Cat. No. 328014)
- Structure
- Integrin family, associates with integrin ?1 (CD29), 135 kD and 25 kD in reduced condition, 155 kD (unreduced)
- Distribution
-
Thymocytes, activated lymphocytes, endothelial cells, osteoblasts, melanoma, some myeloid leukemia cells
- Function
- Adhesion, regulation of cell survival and apoptosis
- Ligand/Receptor
- Fibronectin, neural adhesion molecule L1
- Cell Type
- Endothelial cells, Leukemia, Lymphocytes, Mesenchymal Stem Cells, Osteoclasts, Thymocytes
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1.Tran Van Nhieu GT and Isberg RR. 1991. J. Biol. Chem. 266:24367.
2. Isaji T, et al. 2006.J. Biol. Chem. 281:33258.
- Gene ID
- 3678 View all products for this Gene ID
- UniProt
- View information about CD49e on UniProt.org
