Ultra-LEAF™ Purified anti-human IFN-γ -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2580	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

E. coli - expressed, recombinant human IFN-γ

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

ELISA Capture - Quality tested
ELISPOT Capture, Neut - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1.0 - 4.0 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of IFN-γ recombinant protein ranging from 1000 to 8 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Capture²:?The purified NIB42 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated 4S.B3 antibody (Cat. No. 502504/502514) as the detecting antibody. The Ultra-LEAF? purified antibody is suggested for ELISPOT capture. For ELISPOT capture applications, a concentration range of 0.5 - 2.0 ?g/ml is recommended.
Neutralization¹: The Ultra-LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of human IFN-? bioactivity (Cat. No. 502405 & 502406).
Note: For testing human IFN-? in serum or plasma, BioLegend's ELISA Max? Sets (Cat. No. 430101 to 430106) are specially developed and recommended.

Application References (PubMed link indicates BioLegend citation)

Meager A. 1987. Lymphokines and Interferons:A Practical Approach. IRL Press Ltd, Oxford, p. 105.
Goodier M, et al. 2000. J. Immunol. 165:139.
Blank N, et al. 2007. J. Immunol. 179:3613. (Neut)

RRID

AB_2814395 (BioLegend Cat. No. 502405) AB_2814396 (BioLegend Cat. No. 502406)

Antigen Details

Structure: Cytokine; dimer; 20-25 kD (Mammalian)
Bioactivity: Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Cell Sources: CD8⁺ and CD4⁺ T cells, NK cells
Cell Targets: T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors: IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. De Maeyer, E., et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar, M., et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray, P., et al. 1987. Lymphokines 13:151.
Regulation: Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH2
Gene ID: 3458 View all products for this Gene ID
UniProt: View information about IFN-gamma on UniProt.org

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