Purified anti-human IFN-γ Antibody -上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 996	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

E. coli - expressed, recombinant human IFN-γ

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

ELISA Capture - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA Capture applications, the antibody should be titrated between 0.25 - 2 ?g/ml to determine optimal condition. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Capture²:?The purified NIB42 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated 4S.B3 antibody (Cat. No. 502504/502514) as the detecting antibody. The Ultra-LEAF? purified antibody is suggested for ELISPOT capture. For ELISPOT capture applications, a concentration range of 0.5 - 2.0 ?g/ml is recommended.
Neutralization¹: The Ultra-LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of human IFN-? bioactivity (Cat. No. 502405 & 502406).
Note: For testing human IFN-? in serum or plasma, BioLegend's ELISA Max? Sets (Cat. No. 430101 to 430106) are specially developed and recommended.

Application References (PubMed link indicates BioLegend citation)

Meager A. 1987. Lymphokines and Interferons:A Practical Approach. IRL Press Ltd, Oxford, p. 105.
Goodier M, et al. 2000. J. Immunol. 165:139.
Blank N, et al. 2007. J. Immunol. 179:3613. (Neut)

Product Citations

Galaski J, et al. 2021. Front Cell Infect Microbiol. 11:692544. PubMed
Stein N, et al. 2019. iScience. 0.781944444. PubMed
Wilson AS, et al. 2022. Nat Commun. 13:528. PubMed
Kameda M, et al. 2020. PLoS One. 15:e0241719. PubMed
Lockridge J, et al. 2011. PLoS One. 6:e21701. PubMed

RRID

AB_315222 (BioLegend Cat. No. 502401) AB_315223 (BioLegend Cat. No. 502402)

Antigen Details

Structure: Cytokine; dimer; 20-25 kD (Mammalian)
Bioactivity: Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Cell Sources: CD8⁺ and CD4⁺ T cells, NK cells
Cell Targets: T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors: IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. De Maeyer, E., et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar, M., et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray, P., et al. 1987. Lymphokines 13:151.
Regulation: Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH2
Gene ID: 3458 View all products for this Gene ID
UniProt: View information about IFN-gamma on UniProt.org

上海细胞库

原代细胞

细胞培养

细胞服务

酶联试剂盒

生化试剂盒

PCR试剂盒

抗体蛋白

试剂耗材

首页 > 抗体蛋白 > 抗体

产品详情

Product Details

Antigen Details

联系我们