Purified anti-Neurogranin (NRGN) Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3180	1

Product Details

Verified Reactivity

Human, Mouse, Rat

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

KLH conjugated peptide corresponding to residues 52-78 of human NRGN.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

IHC-P - Quality tested
WB - Verified
IP, ELISA Capture - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 5.0 ?g/ml is suggested.?For Western blotting, the suggested use of this reagent is 1.0 - 10.0 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Tissue Sections: Formalin-fixed, paraffin-embedded tissue sections
Primary Antibody Incubation: 20 minutes at room temperature
Antigen Retrieval: Sodium citrate pH 6 (Cat# 928501)
Positive Control: Rat or human brain tissue

Application Notes

Additional reported applications (for the relevant formats of this clone) include: immunoprecipitation and ELISA capture.

The epitope has been mapped to a.a. 52-63 of human NRGN.

Application References (PubMed link indicates BioLegend citation)

Kvartsberg H, et al. 2014. Alzheimers. Dement. 10:1180-90.

RRID

AB_2566623 (BioLegend Cat. No. 845702)

Antigen Details

Structure: 78 amino acids with a molecular weight of 7.6 kD.
Distribution: In the cerebral cortex, NRGN is found in the cell bodies of neurons in layers II-VI, and in apical and basal dendrites of pyramidal neurons. It is not found in the dendrites in patients with Alzheimer's disease.
Function: Neurogranin is a neuron-specific protein kinase C substrate (post synaptic protein) whose main function is to bind CaM (calmodulin) in the absence of Ca²⁺. Neurogranin modulates the homeostasis of Ca²⁺ in neurons and its role has been implicated in learning and memory.
Ligand/Receptor: Calmodulin-binding.
Cell Type: Neurons
Biology Area: Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family: Postsynaptic proteins
Antigen References: 1. Zhong L, et al. 2015. J. Neurosci. 35:7503.
2. Huang K, et al. 2004. J. Neurosci. 24:10660.
3. Dominguez-Gonzalez I, et al. 2007. Biochem. J. 404:31.
4. Prichard L, et al. 1999. J. Biol. Chem. 274:7689.
Gene ID: 4900 View all products for this Gene ID
UniProt: View information about Neurogranin on UniProt.org

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