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Purified anti-human CD279 (PD-1) Antibody
产品名称:
Purified anti-human CD279 (PD-1) Antibody
产品类别:
抗体
产品编号:
367402
产品应用:
367402
[价格]
规格 价格 库存
100µg ¥ 1344 1

产品详情

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
YT cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P, WB - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?l volume. For immunohistochemistry of paraffin-embedded tissue, a concentration range of 0.1 - 10 ?g/ml is suggested. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 ?g/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Tissue Sections: Paraffin-embedded tissues.
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Sodium Citrate H.I.E.R. Retrieval Solution (Cat. No. 928501, 928601).
Incubation: 24 hours at 2°C - 4 °C.
Positive Control: Human tonsil and spleen.

Application Notes

Additional reported applications (for the relevant formats of this clone) include: immunofluorescence staining.

We recommend using our Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB) (Cat. No. 929501).

Application References

(PubMed link indicates BioLegend citation)
  1. Roncador G, et al. 2007. Haematologica 92:1059.
  2. Nam-Cha SH, et al. 2008. Am. J. Surg. Pathol. 32:1252.
  3. Rodriguez-Pinilla SM, et al. 2009. Am. J. Surg. Pathol. 33:81.
Product Citations
  1. Kim JH, et al. 2020. Foods. 9:00. PubMed
  2. Franchini DM et al. 2019. Cell reports. 26(1):94-107 . PubMed
RRID
AB_2565782 (BioLegend Cat. No. 367402)

Antigen Details

Structure
55 kD type I transmembrane protein.
Distribution

Transiently expressed on CD4- and CD8- thymocytes, upregulated in thymocytes and splenic T and B lymphocytes, and is expressed on activated myeloid cells.

Function
Signaling, co-stimulation (co-inhibition), immunoglobulin superfamily.
Interaction
SHP-1 and SHP-2.
Ligand/Receptor
PD-L1 (CD274) and PD-L2 (CD273).
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Cancer Biomarkers, Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Francisco LM, Sage PT, and Sharpe AH. 2010. Immunological Rev. 236:219.

Gene ID
5133 View all products for this Gene ID
UniProt
View information about CD279 on UniProt.org

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