Purified anti-human CD279 (PD-1) Antibody
- 产品名称:
- Purified anti-human CD279 (PD-1) Antibody
- 产品类别:
- 抗体
- 产品编号:
- 367402
- 产品应用:
- 367402
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- YT cell line.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-P, WB - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?l volume. For immunohistochemistry of paraffin-embedded tissue, a concentration range of 0.1 - 10 ?g/ml is suggested. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 ?g/ml. It is recommended that the reagent be titrated for optimal performance for each application.
Tissue Sections: Paraffin-embedded tissues.
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Sodium Citrate H.I.E.R. Retrieval Solution (Cat. No. 928501, 928601).
Incubation: 24 hours at 2°C - 4 °C.
Positive Control: Human tonsil and spleen.
- Application Notes
Additional reported applications (for the relevant formats of this clone) include: immunofluorescence staining.
We recommend using our Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB) (Cat. No. 929501).
- Application References
(PubMed link indicates BioLegend citation) -
- Roncador G, et al. 2007. Haematologica 92:1059.
- Nam-Cha SH, et al. 2008. Am. J. Surg. Pathol. 32:1252.
- Rodriguez-Pinilla SM, et al. 2009. Am. J. Surg. Pathol. 33:81.
- Product Citations
-
- Kim JH, et al. 2020. Foods. 9:00. PubMed
- Franchini DM et al. 2019. Cell reports. 26(1):94-107 . PubMed
- RRID
- AB_2565782 (BioLegend Cat. No. 367402)
- Structure
- 55 kD type I transmembrane protein.
- Distribution
-
Transiently expressed on CD4- and CD8- thymocytes, upregulated in thymocytes and splenic T and B lymphocytes, and is expressed on activated myeloid cells.
- Function
- Signaling, co-stimulation (co-inhibition), immunoglobulin superfamily.
- Interaction
- SHP-1 and SHP-2.
- Ligand/Receptor
- PD-L1 (CD274) and PD-L2 (CD273).
- Cell Type
- B cells, T cells, Thymocytes, Tregs
- Biology Area
- Cancer Biomarkers, Immunology, Inhibitory Molecules
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Francisco LM, Sage PT, and Sharpe AH. 2010. Immunological Rev. 236:219.
- Gene ID
- 5133 View all products for this Gene ID
- UniProt
- View information about CD279 on UniProt.org