Purified anti-β-Amyloid, 1-11 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3312	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

This antibody was raised against a peptide sequence corresponding to amino acids 1-11 of human amyloid beta (Aβ) protein.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

IHC-P?- Quality tested
ELISA Detection - Verified
IP, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 5.0 ?g/ml is suggested. For ELISA Detection, a concentration range of 25 - 50 ng/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the releavant formats) include immunoprecipication⁴, Western blotting^1,2,4,9, and immunoflurescence microscopy⁷.

NAB 228 recognizes a peptide corresponding to amino acid residues 1-11 of the human amyloid beta (A?) as determined by ELISA using peptides that block its immunoreactivity. This antibody also recognizes full length amyloid precursor protein (APP), soluble APP form (sAPPa), C99 cleavage form, and A? (1-40 and 1-42). NAP 228 does not recognize sAPP?.

This antibody can be used to stain amyloid plaques (diffuse and compact) and amyloid angiopathy on formalin-fixed paraffin-embedded tissue (human AD, Downs syndrome, MCI and transgenic mouse).

Application References (PubMed link indicates BioLegend citation)

Lee EB, et al. 2003. J. Biol. Chem. 278(7):4458-66. (WB) PubMed
Mandler M, et al. 2015. PLoS One 1:e0115237. (WB) PubMed
Wolk DA, et al. 2011. Arch. Neurol. 11:1398. (IHC) PubMed
Lee EB, et al. 2006. J. Biol. Chem. 7:4292. (IP, WB, IHC) PubMed
Berlau DJ, et al. 2013. Alzheimers Dement. 6:699. (IHC) PubMed
Robinson JL, et al. 2014. Brain 137:2578. (IHC) PubMed
Carroll JC, et al. 2011. J. Neurosci. 40:14436. (IHC, IF) PubMed
Hurtado DE, et al. 2010. Am. J. Pathol. 4:1977. (IHC) PubMed
Lee EB, et al. 2005. J. Cell Biol. 2:291. (IHC, WB) PubMed

RRID

AB_2632642 (BioLegend Cat. No. 835104)

Antigen Details

Structure: Amyloid precursor protein is a 770 amino acid protein with a molecular mass of approximately 90 kD. A? denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. A? has an apparent molecular mass of about 4 kD.
Distribution: Cellular distribution: cytosol, extracellular, endosomes, golgi apparatus.
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family: APP/β-Amyloid
Gene ID: 351 View all products for this Gene ID
UniProt: View information about beta-Amyloid, 1-11 on UniProt.org

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