Spark Blue™ 550 anti-mouse CD11c Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1692	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Mouse spleen dendritic cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide

Preparation

The antibody was purified by affinity chromatography and conjugated with Spark Blue? 550 under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC -?Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25??g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Blue? 550 has a maximum excitation of 516 nm and a maximum emission of 540 nm.

Excitation Laser

Blue Laser (488 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation³, immunohistochemical staining of acetone-fixed frozen sections³, immunofluorescence microscopy^{5, 9} (Alexa Fluor? 488 conjugated N418 was used for IHC in frozen sections¹⁰), and spatial biology (IBEX)^22,23.

Application References (PubMed link indicates BioLegend citation)

Granucci F, et al. 1997. J. Immunol. 159:1794.
Stokes RW, et al. 1998. J. Immunol. 160:5514.
Metlay JP, et al. 1990. J. Exp. Med. 171:1753. (IHC, IP)
Ma XT, et al. 2006. Cancer Research 66:1169.
Chin RK, et al. 2006. J. Immunol. 177:290. (IF)
Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC) PubMed
Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC) PubMed
Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
You Y, et al. 2009. J. Immunol. 182:7343. (IF) PubMed
Roland CL, et al. 2009. Mol. Cancer Res. 8:1761. (IHC, FC) PubMed
Wikstrom M, et al.2006. J. Immunol. 177:913. PubMed
Pericolini E, et al. 2008. J. Leukocyte Biol. 83:1286. PubMed
Randall LM, et al. 2008. Infect. Immun.76:3312. PubMed
Fahlen-Yrild L, et al. 2009. J. Immunol. 183:5032. PubMed
Osterholzer JJ, et al. 2009. J. Immunol. 183:8044. PubMed
Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
Eisenach PA, et al. 2010. J Cell Sci. 123:4182. PubMed
Leppin K, et al. 2014. Invest. Ophthalmol. Vis. Sci. 55:3603. PubMed
Sakai F, et al. 2014. PLoS One. 9:105370. PubMed
Gibbins JD, et al. 2014. Blood. 124:2953. PubMed
White CE, et al. 2015. J Immunol. 194:697. PubMed
Lu X, et al. 2015. J Immunol. 194:2011. PubMed
Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

RRID

AB_2860633 (BioLegend Cat. No. 117365) AB_2860634 (BioLegend Cat. No. 117366)

Antigen Details

Structure: Integrin α-chain, associates with integrin β₂ (CD18), 150 kD
Distribution: Dendritic cells, NK cells, intestinal intraepithelial lymphocytes (IEL), some activated T cells
Function: Cellular adhesion
Ligand/Receptor: iC3b, fibrinogen
Cell Type: Dendritic cells, Epithelial cells, NK cells, T cells, Tregs
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lopez-Rodriguez C, et al. 1996. J. Immunol. 156:3780.
Gene ID: 16411 View all products for this Gene ID
UniProt: View information about CD11c on UniProt.org

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