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Brilliant Violet 711™ anti-mouse CD11c Antib
产品名称：
Brilliant Violet 711™ anti-mouse CD11c Antib
产品类别：
抗体
产品编号：
117349
产品应用：
117349
[价格]
规格 价格 库存
50µg ￥ 3432 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse spleen dendritic cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711? under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 711? excites at 405 nm and emits at 711 nm. The bandpass filter 710/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, immunofluorescence microscopy5, 9 (Alexa Fluor? 488 conjugated N418 was used for IHC in frozen sections10), and spatial biology (IBEX)22,23.

Application References

(PubMed link indicates BioLegend citation)
  1. Granucci F, et al. 1997. J. Immunol. 159:1794.
  2. Stokes RW, et al. 1998. J. Immunol. 160:5514.
  3. Metlay JP, et al. 1990. J. Exp. Med. 171:1753. (IHC, IP)
  4. Ma XT, et al. 2006. Cancer Research 66:1169.
  5. Chin RK, et al. 2006. J. Immunol. 177:290. (IF)
  6. Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC) PubMed
  7. Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC) PubMed
  8. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
  9. You Y, et al. 2009. J. Immunol. 182:7343. (IF) PubMed
  10. Roland CL, et al. 2009. Mol. Cancer Res. 8:1761. (IHC, FC) PubMed
  11. Wikstrom M, et al.2006. J. Immunol. 177:913. PubMed
  12. Pericolini E, et al. 2008. J. Leukocyte Biol. 83:1286. PubMed
  13. Randall LM, et al. 2008. Infect. Immun.76:3312. PubMed
  14. Fahlen-Yrild L, et al. 2009. J. Immunol. 183:5032. PubMed
  15. Osterholzer JJ, et al. 2009. J. Immunol. 183:8044. PubMed
  16. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  17. Eisenach PA, et al. 2010. J Cell Sci. 123:4182. PubMed
  18. Leppin K, et al. 2014. Invest. Ophthalmol. Vis. Sci. 55:3603. PubMed
  19. Sakai F, et al. 2014. PLoS One. 9:105370. PubMed
  20. Gibbins JD, et al. 2014. Blood. 124:2953. PubMed
  21. White CE, et al. 2015. J Immunol. 194:697. PubMed
  22. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  23. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  24. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Vasanthakumar A, et al. 2020. Nature. 579:581. PubMed
  2. Daemen S, et al. 2021. Cell Reports. 34(2):108626. PubMed
  3. Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed
  4. Israelow B, et al. 2020. bioRxiv. . PubMed
  5. Ren Z, et al. 2021. EMBO Molecular Medicine. :e14059. PubMed
  6. Kathryn A Pape et al. 2018. Immunity. 48(6):1135-1143 . PubMed
  7. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  8. Jennings EK, et al. 2021. STAR Protocols. 2(1):100284. PubMed
  9. Christopher S Garris et al. 2018. Immunity. 49(6):1148-1161 . PubMed
  10. Goggi JL, et al. 2020. Mol Imaging Biol. 22:1392. PubMed
  11. Jager SE, et al. 2020. Glia. 68(7):1375-1395. PubMed
  12. Yang C, et al. 2022. Research (Wash D C). 2022:9768687. PubMed
  13. Germundson DL, et al. 2022. Front Allergy. 3:870513. PubMed
  14. Aktories P, et al. 2022. Cell Rep Methods. 2:100260. PubMed
  15. Coronel MM, et al. 2020. Sci Adv. 6:eaba5573. PubMed
  16. Daemen S, et al. 2021. STAR Protoc. 2:100511. PubMed
  17. Prizant H, et al. 2021. Cell Reports. 36(6):109523. PubMed
  18. Mossadegh–Keller N, et al. 2019. Bio Protoc. 9:5. PubMed
  19. Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
  20. Yousef H, et al. 2019. Nat Med. 25:988. PubMed
  21. Wang Y, et al. 2020. eLife. 9:e57438.. PubMed
  22. Baratin M, et al. 2017. Immunity. 47:349. PubMed
  23. Hartwig T et al. 2017. Molecular cell. 65(4):730-742 . PubMed
  24. Deets KA, et al. 2021. Elife. 10:. PubMed
RRID
AB_2563905 (BioLegend Cat. No. 117349)

Antigen Details

Structure
Integrin α-chain, associates with integrin β2 (CD18), 150 kD
Distribution

Dendritic cells, NK cells, intestinal intraepithelial lymphocytes (IEL), some activated T cells

Function
Cellular adhesion
Ligand/Receptor
iC3b, fibrinogen
Cell Type
Dendritic cells, Epithelial cells, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lopez-Rodriguez C, et al. 1996. J. Immunol. 156:3780.

Gene ID
16411 View all products for this Gene ID
UniProt
View information about CD11c on UniProt.org

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