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Alexa Fluor® 647 anti-mouse CD11c Antibody
产品名称：
Alexa Fluor® 647 anti-mouse CD11c Antibody
产品类别：
抗体
产品编号：
117314
产品应用：
117314
[价格]
规格 价格 库存
25µg ￥ 1212 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse spleen dendritic cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
3D IHC - Verified
IHC - Reported but collaborator, not verified in house
ICC, SB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?L?volume. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for other applications.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, immunofluorescence microscopy5, 9 (Alexa Fluor? 488 conjugated N418 was used for IHC in frozen sections10), and spatial biology (IBEX)22,23.

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References

(PubMed link indicates BioLegend citation)
  1. Granucci F, et al. 1997. J. Immunol. 159:1794.
  2. Stokes RW, et al. 1998. J. Immunol. 160:5514.
  3. Metlay JP, et al. 1990. J. Exp. Med. 171:1753. (IHC, IP)
  4. Ma XT, et al. 2006. Cancer Research 66:1169.
  5. Chin RK, et al. 2006. J. Immunol. 177:290. (IF)
  6. Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC) PubMed
  7. Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC) PubMed
  8. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
  9. You Y, et al. 2009. J. Immunol. 182:7343. (IF) PubMed
  10. Roland CL, et al. 2009. Mol. Cancer Res. 8:1761. (IHC, FC) PubMed
  11. Wikstrom M, et al.2006. J. Immunol. 177:913. PubMed
  12. Pericolini E, et al. 2008. J. Leukocyte Biol. 83:1286. PubMed
  13. Randall LM, et al. 2008. Infect. Immun.76:3312. PubMed
  14. Fahlen-Yrild L, et al. 2009. J. Immunol. 183:5032. PubMed
  15. Osterholzer JJ, et al. 2009. J. Immunol. 183:8044. PubMed
  16. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  17. Eisenach PA, et al. 2010. J Cell Sci. 123:4182. PubMed
  18. Leppin K, et al. 2014. Invest. Ophthalmol. Vis. Sci. 55:3603. PubMed
  19. Sakai F, et al. 2014. PLoS One. 9:105370. PubMed
  20. Gibbins JD, et al. 2014. Blood. 124:2953. PubMed
  21. White CE, et al. 2015. J Immunol. 194:697. PubMed
  22. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  23. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  24. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Vella JL, et al. 2021. Life Sci Alliance. 4:. PubMed
  2. Kim S, et al. 2020. Immunity. 53(4):759-774.e9. PubMed
  3. Reboldi A, et al. 2016. Sci Transl Med. 352: aaf4822. PubMed
  4. Lei G, et al. 2016. Antimicrob Agents Chemother. 60: 1289 - 1297. PubMed
  5. Groza D, et al. 2018. Oncoimmunology. 7:e1424676. PubMed
  6. Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed
  7. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  8. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  9. Bradford BM, et al. 2018. Parasite Immunol. 40:e12566. PubMed
  10. Castellanos CA, et al. 2021. Sci Immunol. 6:eabh0707. PubMed
  11. Roufaiel M, et al. 2016. Nat Immunol. 10.1038/ni.3564. PubMed
  12. Bates J, et al. 2009. J Immunol. 182:7539. PubMed
  13. Hays C, et al. 2020. eLife. 8:e48772.. PubMed
  14. Shen H, et al. 2022. Nat Commun. 13:6030. PubMed
  15. Haugh KA, et al. 2021. eLife. 0.416666666666667. PubMed
  16. Hagan AS, et al. 2020. Development. 147:00:00. PubMed
  17. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  18. Caronni N, et al. 2018. Cancer Res. 78:1685. PubMed
  19. An S, et al. 2019. Small. 15:e1805182. PubMed
  20. Hildebrand KM, et al. 2021. PLoS One. 16:e0253864. PubMed
  21. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  22. Kishore M et al. 2017. Immunity. 47(5):875-889 . PubMed
  23. Zheng D, et al. 2022. Acta Pharm Sin B. 12:2740. PubMed
  24. Kim SH, et al. 2021. Cell Reports. 35(2):108995. PubMed
  25. Koikawa K, et al. 2021. Cell. 184(18):4753-4771.e27. PubMed
  26. Collins N, et al. 2016. Nat Commun. 7:11514. PubMed
  27. Stevenson ER, et al. 2022. J Pharmacol Exp Ther. 382:356. PubMed
  28. Mambres D, et al. 2016. J Immunol. 196: 3780 - 3793. PubMed
  29. Vacca F, et al. 2020. eLife. 9:e54017.. PubMed
  30. Barbet G, et al. 2018. Immunity. 48:584. PubMed
  31. Shaikh H, et al. 2021. Front Immunol. 12:689896. PubMed
  32. Lindhorst A, et al. 2021. Cell Death Dis. 12:579. PubMed
  33. Chen S, et al. 2015. Blood. 126: 103 - 112. PubMed
  34. Tran NL, et al. 2022. Elife. 11:. PubMed
  35. Baker GJ, et al. 2020. Cell Syst. 0.647222222. PubMed
  36. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  37. Markey KA et al. 2019. Current protocols in immunology. 125(1):e72 . PubMed
  38. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  39. Fox B, et al. 2016. PLoS One. 12: 1006189. PubMed
  40. Song W, et al. 2022. Immunity. 55:290. PubMed
RRID
AB_492850 (BioLegend Cat. No. 117314) AB_389328 (BioLegend Cat. No. 117312)

Antigen Details

Structure
Integrin α-chain, associates with integrin β2 (CD18), 150 kD
Distribution

Dendritic cells, NK cells, intestinal intraepithelial lymphocytes (IEL), some activated T cells

Function
Cellular adhesion
Ligand/Receptor
iC3b, fibrinogen
Cell Type
Dendritic cells, Epithelial cells, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lopez-Rodriguez C, et al. 1996. J. Immunol. 156:3780.

Gene ID
16411 View all products for this Gene ID
UniProt
View information about CD11c on UniProt.org

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