Ultra-LEAF™ Purified anti-mouse CD253 (TRAIL
- 产品名称：
- Ultra-LEAF™ Purified anti-mouse CD253 (TRAIL
- 产品类别：
- 抗体
- 产品编号：
- 109315
- 产品应用：
- 109315
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse TRAIL-transfected 2PK-3 cells
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Qualit tested
Block - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: in vitro blocking of NK cell cytotoxicity1,2. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 109308).
- Application References
(PubMed link indicates BioLegend citation) -
- Kayagaki N, et al. 1999. J. Immunol. 163:1906. (Block)
- Sato K, et al. 2005. J. Immunol. 174:4025. (Block)
- Joshi PS, et al. 2006. J. Leukocyte Biol. 80:1345.
- Herold S, et al. 2008. J. Exp. Med. 205:3065. PubMed
- Iannello A, et al. 2009. J. Virol. 83:5999. PubMed
- Komatsu M, et al. 2003. Blood 101:3991. (Block)
- Taieb J, et al. 2006. Nature Med. 12:214. (Block)
- Gonzaga R, et al. 2011. J. Immunol. 187:6235. PubMed.
- RRID
- AB_2813976 (BioLegend Cat. No. 109315) AB_2813977 (BioLegend Cat. No. 109316)
- Structure
- TNF ligand superfamily member, approximately 40 kD
- Distribution
-
Widespread, highest expression in spleen, lung, prostate; NK, monocytes, neutrophils, activated T cells
- Function
- Induces apoptosis in tumorigenic and transformed cell lines, involved in T cell mediated cytotoxicity
- Ligand/Receptor
- TRAIL receptor DR5
- Cell Type
- Monocytes, Neutrophils, NK cells, T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Kayagaki N, et al. 1999. J. Immunol. 163:1906.
2. Wiley SR, et al. 1995. Immunity. 3:673.
3. Wu GS, et al. 1999. Cancer Res. 59:2770.
4. Mariani SM, et al. 1998. Eur. J. Immunol. 28:973.
- Gene ID
- 22035 View all products for this Gene ID
- UniProt
- View information about CD253 on UniProt.org
