Ultra-LEAF™ Purified anti-Glutamic Acid Deca
- 产品名称：
- Ultra-LEAF™ Purified anti-Glutamic Acid Deca
- 产品类别：
- 抗体
- 产品编号：
- 844503
- 产品应用：
- 844503
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Peptide immunogen corresponding to amino acids 4-22 of human GAD65.
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
WB - Quality tested
IHC-P - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 ?g per ml. For immunohistochemistry formalin-fixed paraffin-embedded tissues, a concentration range of 1.0 - 5.0 ?g/ml is suggested.? It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats of this clone) include: immunoprecipitation.1
For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 844503) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).
This antibody demonstrates weaker reactivity to human compared to mouse GAD65/GAD2
- Application References
(PubMed link indicates BioLegend citation) -
- Hampe CS, et al. 2001. J. Neuroimmunol. 113:63. (IP, WB)
- Hampe CS, et al. 2007. Clin. Exp. Immunol. 148:72.
- Ziegler B, et al. 1996. Acta Diabetol. 33:225.
- Raju R, et al. 2005. J. Immunol. 175:7755.
- RRID
- AB_2565804 (BioLegend Cat. No. 844503)
- Structure
- 585 amino acids with a predicted molecular weight of 65 kD.
- Distribution
-
Cytoplasm, cytoplasmic vesicle, golgi apparatus, plasma membrane, and marker of pre-synaptic terminals.
- Function
- Catalyzes production of GABA from L-glutamic acid.
- Interaction
- Taurine and hypotaurine metabolism, transmission across chemical synapses.
- Cell Type
- GABAergic Neurons
- Biology Area
- Cell Biology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Signal Transduction
- Antigen References
-
1. Walls A, et al. 2011. J. of Cerebral Blood Flow & Metabolism 31:494.
- Gene ID
- 2572 View all products for this Gene ID
- UniProt
- View information about Glutamic Acid Decarboxylase on UniProt.org
