PerCP/Cyanine5.5 anti-mouse CD41 Antibody
- 产品名称：
- PerCP/Cyanine5.5 anti-mouse CD41 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 133917
- 产品应用：
- 133917
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse platelets
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Application Notes
Additional reported applications (for the relevant formats) include: depletion of platelets and functional assay in vivo.4,7 The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for in vivo studies (Cat. No.?133939).
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Nieswandt B, et al. 1999. Blood 94:684.
- Teeling JL, et al. 2001. Blood 98:1095.
- Bertrand JY, et al. 2005. P. Natl. Acad. Sci. USA 102:134.
- Nocito A, et al. 2007. Hepatology 45:369. (Deplete)
- Sullivan BP, et al. 2010. Toxicol. Sci. 115:286. (Deplete) PubMed
- van der Heyde HC, et al. 2005. Blood 105:1956. (FA)
- Marjon KD, et al. 2009. J. Immunol. 182:1397. (Deplete)
- Product Citations
-
- Ruhela D, et al. 2021. Brain Behav Immun Health. 18:100398. PubMed
- Yang M, et al. 2015. J Appl Physiol. 119: 219-222. PubMed
- Labuhn M, et al. 2020. Cancer Cell. 36(2):123-138. PubMed
- Chen X, et al. 2021. Theranostics. 11:4655. PubMed
- Bhopale VM, et al. 2021. J Appl Physiol (1985). 130:1604. PubMed
- Yoshida K, et al. 2022. Sci Rep. 12:17276. PubMed
- Arai F, et al. 2020. Cell Systems. 11(6):640-652.e5. PubMed
- Thom SR, et al. 2017. J Appl Physiol (1985). 123:297. PubMed
- Jiang Y, et al. 2021. Cell Death Differ. 28:3009. PubMed
- Yang M, et al. 2015. J Appl Physiol. 119: 77 - 82. PubMed
- Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
- Thom SR, et al. 2018. J Appl Physiol (1985). 125:1339. PubMed
- Gao Y, et al. 2020. Sci Adv. 6:eaba0588. PubMed
- RRID
- AB_2563499 (BioLegend Cat. No. 133917) AB_2563500 (BioLegend Cat. No. 133918)
- Structure
- A transmembrane glycoprotein that is expressed by platelets and megakaryocytes
- Distribution
-
Platelets, megakaryocytes, and hematopoietic progenitors
- Function
- Associate with CD61 (integrin β3) to form a complex which plays a major role in platelet adhesion and aggregation
- Ligand/Receptor
- Fibrinogen, fibronectin, von Willebrand factor, thrombin
- Cell Type
- Hematopoietic stem and progenitors, Megakaryocytes, Platelets
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Bakewell SJ, et al. 2003. P. Natl. Acad. Sci. USA 100:14205.
2. Phillips DR, et al. 1991. Cell. 65:359.
- Gene ID
- 16399 View all products for this Gene ID
- UniProt
- View information about CD41 on UniProt.org
