Brilliant Violet 421™ anti-mouse CD41 Antibo
- 产品名称：
- Brilliant Violet 421™ anti-mouse CD41 Antibo
- 产品类别：
- 抗体
- 产品编号：
- 133911
- 产品应用：
- 133911
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse platelets
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
- Concentration
- ?g size: 0.2 mg/mL?L size: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the ?l size, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. For flow cytometric staining using the ?g size, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet?.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: depletion of platelets and functional assay in vivo.4,7 The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for in vivo studies (Cat. No.?133939).
- Application References
(PubMed link indicates BioLegend citation) -
- Nieswandt B, et al. 1999. Blood 94:684.
- Teeling JL, et al. 2001. Blood 98:1095.
- Bertrand JY, et al. 2005. P. Natl. Acad. Sci. USA 102:134.
- Nocito A, et al. 2007. Hepatology 45:369. (Deplete)
- Sullivan BP, et al. 2010. Toxicol. Sci. 115:286. (Deplete) PubMed
- van der Heyde HC, et al. 2005. Blood 105:1956. (FA)
- Marjon KD, et al. 2009. J. Immunol. 182:1397. (Deplete)
- Product Citations
-
- Sandovici I, et al. 2022. Dev Cell. 57:63. PubMed
- Jordan KR, et al. 2022. J Thromb Haemost. 20:2035. PubMed
- Yamamoto R et al. 2018. Cell stem cell. 22(4):600-607 . PubMed
- Souilhol C, et al. 2016. Nat Commun. 7:10784. PubMed
- Yeung AK, et al. 2020. Blood Adv. 4:6204. PubMed
- Commerford CD et al. 2018. Cell reports. . PubMed
- Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
- Di Genua C, et al. 2020. Cancer Cell. 37(5):690-704.e8.. PubMed
- Delaney C, et al. 2020. Am J Physiol Lung Cell Mol Physiol. . PubMed
- Mizuno T, et al. 2017. Sci Rep. 7:42714. PubMed
- Marks-Bluth J, et al. 2015. Mol Cell Biol. 35:2165. PubMed
- Fujimoto N, et al. 2020. PLoS Biol. 18:e3000704. PubMed
- Davizon-Castillo P, et al. 2020. Physiol Rep. 8:e14386. PubMed
- Mesnieres M, et al. 2021. Cell Reports. 36(8):109618. PubMed
- Rao T, et al. 2015. Stem Cell Res. 14:307. PubMed
- Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
- Young K, et al. 2021. Cell Stem Cell. . PubMed
- RRID
- AB_10960744 (BioLegend Cat. No. 133911) AB_2650893 (BioLegend Cat. No. 133912)
- Structure
- A transmembrane glycoprotein that is expressed by platelets and megakaryocytes
- Distribution
-
Platelets, megakaryocytes, and hematopoietic progenitors
- Function
- Associate with CD61 (integrin β3) to form a complex which plays a major role in platelet adhesion and aggregation
- Ligand/Receptor
- Fibrinogen, fibronectin, von Willebrand factor, thrombin
- Cell Type
- Hematopoietic stem and progenitors, Megakaryocytes, Platelets
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Bakewell SJ, et al. 2003. P. Natl. Acad. Sci. USA 100:14205.
2. Phillips DR, et al. 1991. Cell. 65:359.
- Gene ID
- 16399 View all products for this Gene ID
- UniProt
- View information about CD41 on UniProt.org
