Ultra-LEAF™ Purified anti-mouse CD255 (TWEAK
- 产品名称：
- Ultra-LEAF™ Purified anti-mouse CD255 (TWEAK
- 产品类别：
- 抗体
- 产品编号：
- 120009
- 产品应用：
- 120009
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC -?Quality tested
Block -?Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0??g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: in vitro blocking of ligand binding2. Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 120009 & 120010).
- Application References
(PubMed link indicates BioLegend citation) -
- Nakayama M, et al. 2003. Biochem. Biophys. Res. Commun. 306:819.
- Mittal A, et al. 2010. J. Cell Biol. 188:833. (Block) PubMed
- RRID
- AB_2860642 (BioLegend Cat. No. 120009) AB_2860643 (BioLegend Cat. No. 120010)
- Structure
- TNF superfamily member, type II membrane protein and secreted (after proteolytic processing); forms homotrimer, 27 kD
- Distribution
-
Widely expressed; induced on activated CD4+ cells
- Function
- Weak inducer of apoptosis in some cell types, activates NF-κB, promotes angiogenesis and endothelial cell proliferation, induces inflammatory cytokine production
- Ligand/Receptor
- TWEAKR/FN14, possible interaction with TNFSF12/APO3
- Cell Type
- T cells
- Biology Area
- Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
- Molecular Family
- CD Molecules, Cytokines/Chemokines
- Antigen References
-
1. Chicheportiche Y, et al. 1997. J. Biol. Chem. 272:32401.
2. Lynch CN, et al. 1999. J. Biol. Chem. 274:8455.
3. Schneider P, et al. 1999. Eur. J. Immunol. 29:1785.
- Gene ID
- 21944 View all products for this Gene ID
- UniProt
- View information about CD255 on UniProt.org
